Macro-Prep 25 Q Media

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Overview

Purification on Macro-Prep 25Q column

Separation of 360 µl of 6xHis-tagged dihydrofolate reductase E. coli lysate on a 2 ml column packed with macro-Prep 25 Q. Buffer A: 59 mM Trist, pH 8.3. Buffer B: 50 mM Trist, pH 8.3, 1 M NaCl; gradient: 0–100% B in 10 column volumes; flow rate: 2 ml/min.

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Macro-Prep 25 Q strong anion exchange support offers high-resolution separations at high flow rates with medium pressures. It possesses the same rigid, macroporous, and hydrophilic properties of 50 µm Macro-Prep High Q support but in a 25 µm bead that offers higher resolution.

Benefits include:

Benchtop to pilot-scale separations, where maximum sample recovery and purity are important
Fewer chromatographic steps required
Removal of protein variants and other difficult contaminants that would require additional validation if present in the final formulation

Type of ion exchanger	Strong anion
Functional group	–N+(CH₃)3
Total ionic capacity	220 ± 40 µeq/ml
Median particle size	25 µm
Nominal pore size	725 Å
Shipping counterion	Cl-
Dynamic binding capacity	>30 mg/ml BSA
Recommended linear flow rate range	50–300 cm/hr
Maximum linear flow rate 3,000 cm.hr	3,000 cm/hr
Chemical stability
1.0 M HCI	>48 hr
100% ethanol	Excellent
1.0 M HCI	Excellent
pH stability	1–10
Volume changes
pH 4–10	<1%
0.1–1.0 M NaCl	<5%
Autoclavability	Yes; do not autoclave the OH- form
Storage solution	20% ethanol