Purification of phosphoinositide- 3-kinase on an UNO Q1 column.
Conditions Buffer A, 40 mM TEA, pH 7.6 Buffer B, buffer A + 1 M KCl, pH 7.6 Gradient, 0–35% B in 20 ml Flow rate, 2.5 ml/min
Data courtesy of Dr A Couvillon, Beth Israel Deaconess Medical Center, Boston, MA USA.
Flow rate comparison for yeast enzyme separation.
Left, 1 ml/min; right, 5 ml/min. Separation on UNO® Q1 column in 20 mM Tris buffer, pH 8.2, with a gradient of 0–1 M NaCl in 20 ml.
UNO monolith ion exchange columns contain a patented* continuous-bed matrix allowing biomolecule separations at high flow rates without sacrificing resolution or capacity. In continuous-bed technology, advanced monomers and ionomers are polymerized directly in the column and aggregate into a dense homogeneous network of nodules. The resulting rigid matrix contains interconnecting channels large enough to allow fast hydrodynamic flow at low backpressures. The continuous-bed matrix yields a column with exceptional resolution, speed, capacity, and value for the separation of biomolecules. The homogeneous, nonporous continuous-bed matrix allows extremely fast mass transfer of proteins and eluent to the ionic functional groups.
UNO Q columns are for strong anion exchange. Choose from the following UNO columns:
* U.S. patent 6,423,666.
720-0001 Pkg of 1, 7 x 35 mm, 1.3 ml, prepacked monolith ion exchange column, 20 mg max. protein load, 0.5–5.0 ml/min flow rate, operating pressure 700 psi
720-0003 Pkg of 1, 12 x 53 mm, 6 ml, prepacked monolith ion exchange column, 90 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
720-0005 Pkg of 1, 15 x 68 mm, 12 ml, prepacked monolith ion exchange column, 180 mg max. protein load, 0.5–8.0 ml/min flow rate, operating pressure 700 psi
720-0009 Pkg of 1, 4.6 x 10 mm, 0.16 ml, prepacked polishing PEEK column, 2 mg max. protein load, 0.1–1.0 ml/min flow rate, operating pressure 200 psi
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