Bio-Scale™ Mini UNOsphere™ Q and UNOsphere S Cartridges

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Overview

Prepacked Cartridge Selection Guide
Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein capacity/ml*
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q UNOsphere™ Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO3- 60 mg human IgG
DEAE Macro-Prep DEAE Weak anion exchange -HN+(C2H5)2 >35 mg protein
Protein Purification
High S Macro-Prep High S Strong cation exchange -SO3- ≥49 mg human IgG
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity Cibacron Blue
F3GA and DEAE		 0.2–1.0 ml serum
Antibody Purification
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse monoclonal IgG1
CHT™ Type I CHT ceramic
hydroxyapatite Type I		 Hydroxyapatite (Ca5(PO4)3OH)2 16–23 mg human IgG
CHT Type II CHT ceramic
hydroxyapatite Type II		 Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA		 0.2 mlm serum
11 mg albumin

*   Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
**  IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

Bio-Scale Mini UNOsphere Q and UNOsphere S cartridges are 5 ml Bio-Scale Mini cartridges filled with either UNOsphere Q or UNOsphere S support:

  • UNOsphere Q — strong anion exchange; chemical form -N+(CH3)3, protein capacity 180 mg/ml BSA
  • UNOsphere S — strong cation exchange; chemical form -SO3- , protein capacity 60 mg/ml human IgG

 

The UNOsphere hydrophophilic polymeric beads are engineered for high mechanical stability and low backpressures. The single-step polymerization produces a support unsurpassed in quality and batch-to-batch reproducibility.

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

 

* U.S.patent 7,208,087.

Sizes (bed volumes) 1, 5, 10, and 50 ml
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Recommended flow rate Varies for each support type*
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions (L x ID)** 1 ml: 40 x 5.6 mm
5 ml: 40 x 12.6 mm
10 ml: 80 x 12.6 mm***
50 ml: 80 x 28.2 mm ***
* Recommended flow rates are given for each support type in the instruction manual.  
** L, length; ID, inner diameter.  
*** Available for desalting cartridges only.  
Bio-Scale Mini UNOsphere Q Cartridges

732-4100
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $190.00
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Bio-Scale Mini UNOsphere Q Cartridge

732-4102
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $135.00
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Bio-Scale Mini UNOsphere Q Cartridges

732-4104
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $465.00
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Bio-Scale Mini UNOsphere S Cartridges

732-4110
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $190.00
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Bio-Scale Mini UNOsphere S Cartridge

732-4112
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $135.00
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Bio-Scale Mini UNOsphere S Cartridges

732-4114
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $465.00
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Number Description Options
2303 Purification of Insulin on UNO S1 Column, Rev A Click to download
2284 Purification of Two Isoforms of Pyruvate Kinase from Nordic Krill Using an UNO Q1 Column, Rev A Click to download
2283 Purification of the Components of Human Macular Carotenoid-Binding Complex, Rev A Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download [ Add to Cart ]