Profinia™ Protein Purification Instrument

The Profinia protein purification instrument is compact and easy to use. Each compartment of the instrument is clearly labeled for fast setup. The buffer compartment allows simple installation of buffers — numbered positions on the instrument match run diagrams and prepackaged reagent labels. The sample compartment is capable of running two samples in series. Both compartments have sipper tubes for the uptake of buffers and samples. The cartridge compartment offers two-step purification capability and uses luer fittings to simplify cartridge connection. Waste collection and diluent bottles are in view and accessible on the sides of the instrument. The fraction collection and sample compartments allow quick installation of standard collection tubes. A central compartment provides convenient storage for a stylus and access to a USB flash drive, which allows you to export run data.

The instrument has a large touch-screen user interface, where you can select options — by finger or stylus — to navigate from screen to screen. Intuitive, user-friendly help functions guide you through the setup and post-run functions of preprogrammed optimized purification methods. The methods and buffer sets include cleaning protocols that maintain the system and prepare the cartridges for future use, ensuring that the Profinia system stays in optimal running condition. This combination of features allows you to set up quickly, walk away, and come back to results in as little as 30 min.

• Optimized methods, cartridges, and buffer kits eliminate time spent on method development, troubleshooting, and reagent preparation
• Built-in reproducibility is achieved with automated system pumps, UV and conductivity detectors, and programmed cleaning methods
• Histidine (His)-tagged, GST-tagged, affinity, and desalting/buffer exchange methods deliver purity and yield comparable to other techniques, and require only a fraction of the time to obtain protein ready for experiments
• Automatic UV peak detection diverts eluted target protein from cartridges to a fraction collection tube for unattended operation
• Easy-access fraction collection and sample compartments hold 15 or 50 ml conical collection tubes
• Benchtop or coldroom operation allows you to control purification conditions; optional cooling accessory keeps samples and fractions cold for benchtop operation
• Additional capabilities let you work with a pH monitor that runs in real time with Profinia software