Prepacked Gravity Flow Columns for Ion Exchange

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Overview

Specifications

Specifications
Catalog # Description Particle Size (µm) Ionic Form Application
731-6211 Poly-Prep Columns AG1-X8 resin, 100–200 mesh, 50 106–180 Chloride Separation of low molecular weight inorganic ions
731-6212 Poly-Prep Columns AG1-X8 resin, 200–400 mesh, 50 45–106 Chloride For high-resolution random separations
731-6221 Poly-Prep Columns AG1-X8 resin, 200–400 mesh, 50 45–106 Formate Separation of low molecular weight biological compounds such as nucleotides, peptides and caarboxylic acids
731-6213 Poly-Prep Columns AG50W-X8 resin, 100–200 mesh, 50 106–250 Hydrogen Separation and concentration of low molecular weight cations such as small peptides and amino acids
731-6214 Poly-Prep Columns AG50W-X8 resin, 200–400 mesh, 50 63–150 Hydrogen For high-resolution random separations
731-1555 Poly-Prep Column Stack Cap. 50     Allows connection of pumps, reservoirs, or columns in series
Prepacked Cartridge Selection Guide
Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein capacity/ml*
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q UNOsphere™ Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO3- 60 mg human IgG
DEAE Macro-Prep DEAE Weak anion exchange -HN+(C2H5)2 >35 mg protein
Protein Purification
High S Macro-Prep High S Strong cation exchange -SO3- ≥49 mg human IgG
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity Cibacron Blue
F3GA and DEAE		 0.2–1.0 ml serum
Antibody Purification
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse monoclonal IgG1
CHT™ Type I CHT ceramic
hydroxyapatite Type I		 Hydroxyapatite (Ca5(PO4)3OH)2 16–23 mg human IgG
CHT Type II CHT ceramic
hydroxyapatite Type II		 Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA		 0.2 mlm serum
11 mg albumin

*   Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
**  IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

Chemical Compatibility
Chemical Compatibility
Chemical Compatibility of Column
Chemical Compatibility*
Acetic acid, 50% S
Aceton S
Acetonitrile S
Ammonium hydroxide S
Chloracetic acid S
Chromic acid, 50% U
Diethyl ether L
Dimethyl formamide S
Dimethylsulfoxide S
Ethyl alcohol S
Ethylene glycol S
Formamide S
Glacial acetic acid S
Glycerine S
Hydrochloric acid, 35% S
Isobutyl alcohol S
Isopropyl alcohol S
Methanol S
Perchloric acid U
Phosphoric acid S
Propylene glycol S
Sodium hydroxide, 50% to Sat. L (U for anions)
Sulfuric acid, 60% S (U for anions)
Sulfuric acid, 98% U
Urea, 8M S

* S = Satisfactory U = Unsatisfactory L = Low

Ion Exchange Capacity
Ion Exchange Capacity
Resin Ion Exchange Capacity
(nominal meq/2 ml of resin)
 
AG® 1-X8 resin 2.4
AG 2-X8 resin 2.4
AG 50W-X4 resin 2.2
AG 50W-X8 resin 3.4
Chelex® 100 resin 0.8
Bio-Rex® 70 resin 4.8
Working Temperature
Working Temperature
Resin Maximum Temperature (°C)
AG® 50 resin 150
AG 1 resin 150 (50°C if converted to OH- form)
AG 2 resin 150 (30°C if converted to OH- form)
Chelex® 100 resin 75
Bio-Rex® 70 resin 100



Poly-Prep® prepacked columns for gravity-flow chromatography provide convenience for sample preparation and other small-scale applications. The graduated 0.8 x 4 cm columns are constructed of polypropylene. Each column holds a standard bed volume of 2 ml AG® ion exchange resin and includes an integral 10 ml reservoir. A Poly-Prep stack cap, which allows connection to pumps, reservoirs, or columns in series, is also available.

 

Poly-Prep Columns AG 1-X8

731-6211
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 1-X8 resin, 100–200 mesh, 106–180 particle size, chloride form, 10 ml reservoir

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Poly-Prep Columns AG 1-X8

731-6212
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 1-X8 resin, 200–400 mesh, 45–106 particle size, chloride form, includes 10 ml reservoir

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Poly-Prep Columns AG 50W-X8

731-6221
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 1-X8 resin, 200–400 mesh, 45–106 particle size, formate form, includes 10 ml reservoir

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Poly-Prep Columns 50W-X8

731-6213
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 50W-X8 resin, 100–200 mesh, 106–250 particle size, hydrogen form, includes 10 ml reservoir

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Poly-Prep Columns AG 50W-X8

731-6214
Pkg of 50, 9 cm high, 2 ml of prepacked analytical grade 50W-X8 resin, 200–400 mesh, 63–150 particle size, hydrogen form, includes 10 ml reservoir

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Poly-Prep Column Stack Cap

731-1555
Pkg of 50, column stack cap, for use with Poly-Prep columns

List Price:   $40.00
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Number Description Options
LIT84 Instruction Manual, Prefilled Poly-Prep Columns for Ion Exchange Chromatography Click to download