Sso7d Fusion Protein Technology
Bio-Rad’s patented* Sso7d fusion protein technology was first employed in the
iProof™ high fidelity DNA polymerase product line. The unique Sso7d fusion polymerase in the SsoFast™ supermixes enables fast cycling without affecting PCR sensitivity, efficiency, and reproducibility. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, increases processivity, and provides greater speed and reduced reaction times compared to conventional DNA polymerases. Furthermore, this polymerase is significantly more resistant to PCR inhibitors, making the SsoFast supermixes ideal choices for applications such as direct qPCR, without the need for sample preparation.
Fusing the double-stranded DNA binding protein Sso7d to DNA polymerases provides a powerful sliding grip on the replicated DNA.
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.
Broad Dynamic Range Under Fast Cycling Conditions
The fusion polymerase in SsoFast™ EvaGreen® supermix with low ROX demonstrates a broad dynamic range under fast cycling conditions using the ABI 7500 fast real-time PCR system.
SsoFast EvaGreen supermix with low ROX generates consistent qPCR results on the ABI 7500 fast real-time PCR system. Tenfold serial dilutions from 10 ng to 100 fg of cDNA were used in each 20 µl reaction. A, cDNA from human spleen was used to detect α-tubulin (α-tubulin efficiency = 105.8%, r = 0.996; total qPCR run time = 39 min, not including melt curve); B, cDNA from human liver was used to detect TIP (TIP efficiency = 92.7%, r = 0.992; total qPCR run time = 42 min, not including melt curve).
Broad Dynamic Range Over Six Orders of Magnitude
SsoFast™ EvaGreen® supermix with low ROX generates linear results over six orders of magnitide on the ABI 7500 fast real-time PCR system. Tenfold serial dilutions of 100 ng to 100 fg of cDNA from HeLa total RNA were used in each 20 µl reaction to detect 18S rRNA. 18S rRNA efficiency = 98.6%, r2 = 0.999.
