SsoFast™ Probes Supermix with ROX

print
SsoFast Probes Supermix with ROX

Overview

Simplex qPCR from cDNA 
Simplex qPCR Results from cDNA

SsoFast™ probes supermix with ROX demonstrates excellent sensitivity and efficiency for simplex qPCR from cDNA using an ABI 7900HT fast real-time PCR system (384-well standard block). cDNA from HeLa cells (100 ng to 0.1 pg) was used in a 10 μl reaction. A, simplex reaction using a VIC-labeled tubulin probe (R2 = 0.9966, efficiency = 92.2%, slope = –3.53); B, simplex reaction using a JOE-labeled cyclin probe (R2 = 0.9976, efficiency = 98.0%, slope = –3.36).

Simplex qPCR from gDNA 
Simplex qPCR Results from gDNA

SsoFast™ probes supermix with ROX demonstrates excellent sensitivity and efficiency for simplex qPCR from genomic DNA using an ABI 7900HT fast real-time PCR system (384-well standard block). Human gDNA (250 ng to 2.5 ng) was used in a 10 μl reaction. A, simplex reaction using a TAMRA-labeled IL-1β probe (R2 = 0.9925, efficiency = 103.9%, slope = –3.23); B, simplex reaction using a TET-labeled CBP probe for a 70.9% GC-rich amplicon (R2 = 0.9959, efficiency = 92.7%, slope = –3.51).

Duplex qPCR from cDNA 
Duplex qPCR Results from cDNA

SsoFast™ probes supermix with ROX demonstrates excellent sensitivity and efficiency for duplex qPCR from targets with approximately a 1,000-fold difference in expression levels using an ABI 7900HT fast real-time PCR system (96-well standard block). cDNA from HeLa cells (100 ng to 0.1 pg) was used in a 20 μl reaction. Duplex reaction using a FAM-labeled TFRC probe (; R2 = 0.9970, efficiency = 90.3%, slope = –3.58) and a VIC-labeled 18S probe (; R2 = 0.9990, efficiency = 88.6%, slope = –3.63).

SsoFast probes supermix with ROX is a ROX-blended version of the high-performing SsoFast probes supermix. It employs patented* Sso7d fusion protein technology for use in probe-based real-time quantitative PCR assays. Its combination of the Sso7d fusion protein technology and an optimized formulation helps perform fast duplex qPCR on Applied Biosystems 7900HT and 7900HT fast real-time PCR systems while maintaining a broad dynamic range, reliability, and accurate quantification for robust gene expression analysis.

  • Fast qPCR results — Sso7d fusion polymerase's stringent hot-start and rapid polymerization kinetics provide fast results in less than 30 min
  • Universal conditions — fully compatible with your pre-existing cycling protocols and primer/probe conditions without the need for additional optimization
  • Minimal PCR inhibition — highly tolerant polymerase exhibits low PCR inhibition for better reproducibility
  • Distinct target detection — robust, simultaneous detection of up to 2 different gene targets using fluorogenic probes under fast qPCR conditions

SsoFast EvaGreen supermix and SsoFast EvaGreen supermix with low ROX, other members of the SsoFast family, also utilize patented* Sso7d fusion protein technology to deliver excellent performance in a wide range of qPCR applications.

* U.S. patents 6,627,424 and 7,541,170.

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 6 months
Customer guarantee (minimum usage time) 12 months
SsoFast™ Probes Supermix with ROX

172-5250
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, enhancers, stabilizers, for 200 x 20 µl reactions

List Price:   $145.00
Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
SsoFast™ Probes Supermix with ROX

172-5251
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, enhancers, stabilizers, for 500 x 20 µl reactions

List Price:   $325.00
Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
SsoFast™ Probes Supermix with ROX

172-5252
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, enhancers, stabilizers, for 1,000 x 20 µl reactions

List Price:   $625.00
Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
SsoFast™ Probes Supermix with ROX

172-5253
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, enhancers, stabilizers, for 2,000 x 20 µl reactions

List Price:   $1,195.00
Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart ]
6051 SsoFast Probes Supermix with ROX Flier, Rev A Click to download
10019663 SsoFast Probes Supermix With ROX Product Insert, Rev A Click to download