iScript™ One-Step RT-PCR Kit With SYBR® Green

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Overview

Broad Dynamic Range
Broad Dynamic Range

The iScript™ one-step RT-PCR kit with SYBR® Green provides high sensitivity across a broad range of concentrations. One-step RT-PCR reactions were performed in triplicate, along with no-template controls, using GAPDH primers and 100 ng to 100 fg of total HeLa RNA. Reactions were carried out on the iCycler iQ® real-time system. Standard curve r = 1.000, slope = -3.466, efficiency = 95%, RFU, relative fluorescence units.

Exceptional Specificity
Exceptional Specificity

Melt-curve analysis of RT-PCR reactions performed using the iScript™ one-step RT-PCR kit with SYBR® Green shows no nonspecific PCR artifacts. Reactions were performed as for the figure above. The melt-curve protocol was performed after the RT-PCR, revealing a single, clean melting peak for all samples tested.

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iScript™ Reverse Transcription Supermix for RT-qPCR iScript™ Advanced cDNA Synthesis Kit for RT-qPCR iScript™ cDNA Synthesis Kit iScript™ Select cDNA Synthesis Kit iScript™ One-Step RT-PCR Kit With SYBR® Green iScript™ One-Step RT-PCR Kit for Probes

The iScript™ one-step RT-PCR kit with SYBR® Green is a highly sensitive solution for real-time quantitative RT-PCR on a broad range of optical thermal cyclers. cDNA synthesis and qPCR (with SYBR® Green) are conveniently carried out in the same tube, using the powerful combination of iScript RNase H+ MMLV reverse transcriptase and hot-start iTaq™ DNA polymerase.

Key Features and Benefits

  • Maximize throughput and reduce the contamination risk of RT-qPCR analysis — 1-step RT-qPCR protocol allows quick and easy screening of a large number of samples
  • Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (100 ng–1 pg) with a highly efficient RNase H+ MMLV-RT
  • Experience superior sensitivity with efficiency with SYBR® Green — formulated for easy optimization of SYBR® Green chemistry.
  • Prevent nonspecific amplification and primer dimers — iTaq antibody-mediated hot-start DNA polymerase contains an antibody that sequesters activity prior to the initial PCR denaturation step
  • Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription
Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
iScript™ One-Step RT-PCR Kit with SYBR® Green

170-8892
50 x 50 μl reactions, one-step real-time RT-PCR kit, includes iScript reverse transcriptase, 2x SYBR® Green RT-PCR reaction mix, nuclease-free water

List Price:   $185.00
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iScript™ One-Step RT-PCR Kit with SYBR® Green

170-8893
200 x 50 μl reactions, one-step real-time RT-PCR kit, includes iScript reverse transcriptase, 2x SYBR® Green RT-PCR reaction mix, nuclease-free water

List Price:   $675.00
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Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart ]
4106269 Instruction Manual, iScript One-Step RT-PCR Kit with SYBR Green, Rev E Click to download
3066 iScript One-Step RT-PCR Kit Flier, Rev C Click to download [ Add to Cart ]
5438 Convenient Multiplex PCR Assays With iQ Multiplex Powermix, Rev A Click to download