iTaq™ Universal SYBR® Green Supermix

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Overview

Superior Gene Expression Analysis
Superior Gene Expression Analysis
Superior Gene Expression Analysis

iTaq™ universal SYBR® Green supermix provides reliable gene expression data. The human β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using a CFX96™ real-time PCR detection system. iTaq™ universal SYBR® Green supermix produced greater than 90% efficiency over 6 orders of linear dynamic range. Standard curve R2 = 0.999, β-actin efficiency = 92.6%, slope =
–3.51. RFU, relative fluorescence units.

Amplification of Genomic DNA
Sensitive and Efficient Amplification of Genomic DNA
Superior Gene Expression Analysis

iTaq™ universal SYBR® Green supermix allows robust amplification of genomic DNA. The human GAPDH gene was amplified from human genomic DNA (50 ng–5 pg) using a CFX96™ real-time PCR detection system. iTaq™ universal SYBR® Green supermix produced a GAPDH efficiency of 108.1% over several orders of linear dynamic range. Standard curve R2 = 0.993, slope = –3.14. RFU, relative fluorescence units.

Consistent Results across Platforms Consistent Results across Platforms
Consistent Results across Real-Time Instrument Platforms
Consistent Results Across Platforms

 

Sensitive and efficient gene expression analysis on any real-time PCR instrument. The β-actin gene was amplified from HeLa cDNA (100 ng–100 fg) using iTaq™ universal SYBR® Green supermix on several real-time instruments. A, Bio-Rad® CFX96™ real-time PCR detection system; B, Bio-Rad® iQ™5 real-time PCR detection system; C, ABI 7900 real-time PCR system; D, ABI 7500 fast real-time PCR system; E, ABI ViiA7 real-time PCR system; F, ABI StepOnePlus real-time PCR system. This supermix showed excellent β-actin efficiencies, R2, and slopes on all platforms (>90% efficiency, R2 > 0.990, slope between –3.20 and –3.58). RFU, relative fluorescence units.

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iTaq™ universal SYBR® Green supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, SYBR® Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and fluorescein).

iTaq™ universal SYBR® Green supermix lets you:

iTaq™ universal SYBR® Green supermix contains:

  • Advanced buffer formulation with SYBR® Green I for optimal qPCR performance
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
  • Blend of passive reference dyes for universal real-time instrument compatibility

Applications and Uses

  • Gene expression analysis
  • Genotyping
  • Mutation detection
  • Pathogen detection

Storage at –20°C Guaranteed for 12 months protected from light in a constant temperature freezer
Repeated freeze/thaw cycles Up to 10 times
Storage at 4°C Guaranteed for 1 month protected from light
Polymerase iTaq DNA polymerase
Hot-start mechanism Antibody mediated
Hot-start activation 95°C, 30 sec
Supermix concentration 2x
Thermal cycling protocol Standard and fast
Instrument compatibility Validated on all Bio-Rad and ROX-dependent Applied Biosystems real-time PCR instruments and Roche LightCycler 480, QIAGEN Rotor-Gene Q, Eppendorf Mastercycler ep realplex, and Stratagene Mx real-time PCR systems
Template compatibility cDNA, genomic DNA, plasmid DNA
Detection method SYBR® Green
iTaq™ Universal SYBR® Green Supermix

172-5120
2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes, for 200 x 20 µl reactions

List Price:   $120.00
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iTaq™ Universal SYBR® Green Supermix

172-5121
5 ml (5 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes, for 500 x 20 µl reactions

List Price:   $260.00
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iTaq™ Universal SYBR® Green Supermix

172-5122
10 ml (10 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes, for 1,000 x 20 µl reactions

List Price:   $500.00
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iTaq™ Universal SYBR® Green Supermix

172-5124
25 ml (5 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes, for 2,500 x 20 µl reactions

List Price:   $1,160.00
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iTaq™ Universal SYBR® Green Supermix

172-5125
50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes, for 5,000 x 20 µl reactions

List Price:   $2,205.00
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Number Description Options
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download
10023652 iTaq Universal SYBR Green Supermix Product Insert, Rev C Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart ]