Macro-Prep Weak Cation Exchange Media

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Overview

Property	DEAE	High Q	High S	CM
Type of support	Weak anion	Strong anion	Strong cation	Weak cation
Functional ligand	–N⁺(C₂H₅)₂	–N⁺(CH₃)₃	–SO₃^-	–COO^-
Ionic capacity	175 ±75 µeq/ml	400 ±75 µeq/ml	160 ±40 µeq/ml	210 ±40 µeq/ml
Typical binding capacity	35 mg BSA/ml	40 mg BSA/ml	70 mg IgG/ml	35 mg hemoglobin/ml
Counterion	Cl^-	Cl^-	Na⁺	Na⁺
Nominal particle size	50 µm	50 µm	50 µm	50 µm
Nominal pore size	1,000 Å	1,000 Å	1,000 Å	1,000 Å
Recommended maximum linear flow rate	3,000 cm/hr	3,000 cm/hr	3,000 cm/hr	3,000 cm/hr
Chemical stability
1% SDS, 24 hr	Yes	Yes	Yes	Yes
6 M guanidine-HCl, 24 hr	Yes	Yes	Yes	Yes
Volume changes
pH 4–10	<1%	<1%	<3%	<1%
0.1–1.0 M NaCl	<5%	<5%	<9%	<4%
Autoclavability* (121°C, 80 min)	Yes	Yes	Yes	Yes
pH stability	1–10	1–10	1–12	1–12
Antimicrobial agent	20% ethanol	20% ethanol	20% ethanol	20% ethanol
Regeneration	1 M NaCl	1 M NaCl	1 M NaCl	1 M NaCl

* Do not autoclave the OH- form.

Product Selection by Technique

		Scale			Packaging Format
Characteristics	Product Type	Analytical	Pilot/Preparative	Process	Prepacked	Bottled
Ion Exchange Chromatography
Separation by charge	UNOsphere™ media					UNOsphere Q, S, Rapid S
General biomolecules purification	Macro-Prep^® media					Macro-Prep High Q, DEAE, High S, CM
Applicable to all antibody purifications	Macro-Prep 25 media					Macro-Prep 25 Q, 25 S
	UNO^® columns				UNO Q, S
	Bio-Scale™ Mini Cartridges				UNOsphere Q, S
High capacity					Macro-Prep High Q, S
Good capture technique	AG^® resins					AG
	Chelex^® resins					Chelex
	Bio-Rex^® resins					Bio-Rex
Hydroxyapatite and Fluoroapatite Chromatography
Unique selectivity	CHT™ 80 µm support					CHT, Types I and II
Alternative to protein A for antibody purification	CHT 40 µm support					CHT, Types I and II
	CHT 20 µm support					CHT, Types I and II
	CFT 40 µm support					CFT, Types I and II
Removal of leached protein A, aggregates, endotoxins, and DNA	Bio-Gel^® HT and HTP media					Bio-Gel HT, HTP
	Bio-Scale columns				CHT Type I (10 µm)
					CHT Type II (20 µm)
Affinity Chromatography
Purification of His- and GST- tagged proteins	Profinity™ IMAC and GST media				Bio-Scale Mini	Profinity IMAC
Separation by biological interaction	Affi-Gel resins					Affi-Gel^® Protein A*
						Affi-Gel Blue*
						DEAE Affi-Gel Blue*
						CM Affi-Gel Blue*
High selectivity						Affi-Gel boronate, heparin**
High purity in a single step						Affi-Gel Hz, 10, 15**
						Affi-Gel 102**
Excellent capture technique	Profinity epoxide media					Profinity epoxide**
	Affi-Prep media					Affi-Prep protein A*
						Affi-Prep polymyxin*
	Bio-Scale Mini cartridges				Profinity IMAC and GST
	Econo-Pac^® columns				Affi-Gel Blue
					DEAE Affi-Gel Blue
					Affi-Gel protein A
Size Exclusion Chromatography
Separation by size	Bio-Gel P media					Bio-Gel P
MW determination	Bio-Beads™ S-X media					Bio-Beads S-X
Applicable to all antibody purifications	Bio-Sil^® and Bio-Silect™ columns				Bio-Sil, Bio-Silect
Desalting or buffer exchange	Bio-Scale Mini cartridges				Bio-Gel P-6
High resolution	Econo-Pac columns				Econo-Pac 10DG
Hydrophobic Interaction Chromatography
Separation by hydrophobicity	Macro-Prep HIC media					Macro-Prep t-butly
						Macro-Prep methyl

* Ready to use.
** Activated.

Common Buffers

Common Buffers for Ion Exchange

Type of Ion Exchanger	Buffer	Buffering Range
Cation	Acetic acid	4.8–5.2
	Citric acid	4.2–5.2
	HEPES	6.8–8.2
	Lactic acid	3.6–4.3
	MES	5.5–6.7
	MOPS	6.5–7.9
	Phosphate	6.7–7.6
	PIPES	6.1–7.5
	TES	7.2–7.8
	Tricine	7.8–8.9
Anion	Bicine	7.6–9.0
	Bis-Tris	5.8–7.2
	Diethanolamine	8.4–8.8
	Diethylamine	9.5–11.5
	L-histidine	5.5–6.0
	Imidazole	6.6–7.1
	Pyridine	4.9–5.6
	Tricine	7.4–8.8
	Triethanolamine	7.3–8.3
	Tris	7.5–8.0

Note:Buffer solution of 50 mM is recommended.

Macro-Prep CM weak cation exchange support meets the demands of analytical, semipreparative, and process-scale applications. This rigid, macroporous hydrophilic support provides exceptional capacity, resolution, and throughput in a chemically and mechanically stable form. Macro-Prep CM operates well at low and medium pressures and exhibits minimal volume changes in response to variations in pH or ionic strength. It provides consistent and reproducible results. Advantages of Macro-Prep CM support include:

High capacity for biomolecules
High resolution of complex biological mixtures
Rigid polymer matrix that allows high flow rates at moderate pressures
Hydrophilic matrix that reduces nonspecific binding
Macroporous beads that enhance access to ionic sites
Chemical, mechanical, and thermal stability