Pulsed Field Standards and Agaroses

Certified megabase agarose — With very high gel strength, high electrophoretic mobility, and high exclusion limit, Certified megabase agarose is the superior choice for CHEF and FIGE applications. The gels remain easy to handle at concentrations as low as 0.3% and allow shorter run times. The separation range is between 1 kb and 5 Mb. Low background staining also provides superior imaging of high molecular weight DNA. Certified megabase agarose has superior electrophoretic mobility, faster migration rates, faster running times, and better separation of megabase fragments than pulsed field Certified agarose.

Pulsed field Certified™ agarose — This agarose enables excellent separation and resolution of large DNA in pulsed field gel applications. The optimal separation range is 1 kb–2 Mb. Although gels cast with this agarose have longer PFGE run times than Certified megabase agarose, running conditions for this agarose are a preset selectable method of the CHEF Mapper XA system auto-algorithm.

Pulsed field standards — Standards for pulsed field applications save days of preparation time. Bio-Rad offers standards for applications from FIGE separation of cosmid inserts to separation of the largest chromosomes. The higher molecular weight standards are prepared in low-melt agarose blocks that can be cut to fit most well dimensions.