ProteinChip Calibration Standards

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Overview

SELDI Citations Database
SELDI Citations
SELDI Citations Database
 

SELDI Citations Database

Request a Biomarker Discovery Guide
Request a Biomarker Discovery Guide

Biomarker Discovery Using SELDI Technology

Proper study and experimental design coupled with the correct data analysis approach are critical components of successful biomarker discovery, no matter which technology, platform, or workflow is used. Bio-Rad has produced two in-depth guides to provide researchers with strategies for success.

The first guide, Biomarker Discovery Using SELDI Technology — A Guide to Successful Study and Experimental Design, provides a series of general guidelines for effective study design using virtually any proteomics technology, and gives specific recommendations for using the ProteinChip surface-enhanced laser desorption/ionization (SELDI) system. This guide also includes:

  • Guidelines for proper sample selection, collection, handling, and storage
  • Instructions for planning the experimental workflow
  • Tips on improving reproducibility through standard operating procedures
  • Important considerations for data analysis implementation
  • Optimized methods and protocols for serum or plasma analysis


The second guide, Biomarker Discovery Using SELDI Technology — A Guide to Data Processing and Analysis Using ProteinChip Data Manager Software, is a comprehensive resource for data analysis workflows, guidelines, and specific recommendations for analyzing biomarker discovery data. The guide covers:

  • General data analysis workflows
  • Data organization
  • Data processing steps, including baseline subtraction, normalization, and spectrum alignment
  • Statistical analysis using P values and ROC curves, hierarchical clustering, and principal component analysis
  • Glossary of software terms

To help ensure accurate profiling and quantitation, a variety of standards and calibrants are available for use with the ProteinChip SELDI system:

  • ProteinChip all-in-one peptide standard — ensures accurate peptide mass assignments; includes vasopressin (human Arg8; 1.08 kD), somatostatin (human; 1.64 kD), dynorphin (porcine; 2.15 kD), ACTH (1–24; human; 2.93 kD), insulin β-chain (bovine; 3.50 kD), insulin (human; 5.81 kD), and recombinant hirudin (6.96 kD)
  • ProteinChip all-in-one protein standard II — ensures accurate protein mass assignments; includes recombinant hirudin (7.0 kD), cytochrome C (bovine; 12.2 kD), myoglobin (equine; 17.0 kD), carbonic anhydrase (bovine red blood cells; 29.0 kD), enolase (S. cerevisiae; 46.7 kD), albumin (bovine; 66.4 kD), and IgG (bovine; 147.3 kD)
  • ProteinChip peptide calibrant kit — includes 7 peptide standards packaged in separate tubes (2 of each standard: 1 nmol each of human angiotensin I, [human Glu1]–fibrinopeptide B, porcine dynorphin A (209–225), human ACTH (1–24), human β-endorphin (61–91), bovine insulin, and bovine ubiquitin)
  • ProteinChip protein calibrant kit — includes 10 lyophilized protein standards packaged in separate tubes (2 of each standard: 1 nmol each of bovine insulin, ubiquitin, cytochrome C, β-lactoglobulin A, superoxide dismutase, and equine myoglobin; 2 nmol each of horseradish peroxidase, bovine serum albumin, and chicken conalbumin; and 5 nmol bovine IgG)
  • ProteinChip human serum control — used as a standard in serum profiling studies
ProteinChip All-In-One Peptide Standard

C10-00005
Pkg of 1, lyophilized mixture of Arg8-vasopressin, somatostatin, dynorphin, ACTH, insulin β-chain, insulin, and recombinant hirudin, for 100 spots
List Price:   $375.00
Quantity:   Add to Quote
 
ProteinChip All-In-One Protein Standard II

C10-00007
Pkg of 1, lyophilized mixture of recombinant hirudin, cytochrome C, myoglobin, carbonic anhydrase, enolase, albumin, and IgG proteins, for 100 spots
List Price:   $375.00
Quantity:   Add to Quote
 
ProteinChip Peptide Calibrant Kit

C10-00002
Pkg of 14 vials, 2 each of angiotensin I, [Glu1]-fibrinopeptide B, ACTH, β-endorphin, insulin, ubiquitin, and dynorphin A
List Price:   $375.00
Quantity:   Add to Quote
 
ProteinChip Protein Calibrant Kit

C10-00001
Pkg of 20 vials, 2 each of insulin, ubiquitin, cytochrome C, β-lactoglobulin A, SOD, myoglobin, HRP, albumin, conalbumin, and IgG
List Price:   $375.00
Quantity:   Add to Quote
 
Number Description Options
5530 ProteinChip SELDI System Brochure, Rev A Click to download
5524 ProteinChip Arrays and Reagents Product Information Sheet, Rev C Click to download
10008421 Instruction Manual, ProteinChip Human Serum Control, Rev B Click to download
10008420 Instruction Manual, ProteinChip Peptide Calibrant Kit, Rev B Click to download
10008419 Instruction Manual, ProteinChip Protein Calibrant Kit, Rev B Click to download
10008244 Instruction Manual, ProteinChip All-in-One Peptide Standard, Rev H Click to download
10013904 Instruction Manual, ProteinChip Peptide Mass Calibration Kit, Rev A Click to download
10008238 Instruction Manual, ProteinChip All-in-One Protein Standard II, Rev G Click to download