Bio-Spin 6 and Micro Bio-Spin 6 Columns

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Overview

Specifications
Specifications
  Bio-Spin 6 Micro Bio-Spin 6 Bio-Spin 30 Micro Bio-Spin 30
Packed support Special grade Bio-Gel P-6 gel Special grade Bio-Gel P-6 gel Special-grade Bio-Gel P-30 gel Special-grade Bio-Gel P-30 gel
Equilibration buffer SSC-buffer* 10 mMTris, pH 7.4 or SSC buffer* SSC buffer* 10 mM Tris, pH 7.4 or SSC buffer*
Applications Desalting and buffer exchange Desalting and buffer exchange Desalting; nucleotide and small molecule removal DNA sequencing reaction mixtures (Tris), and small molecule removal
Bed volume 1.1 ml 0.7 ml 1.1 ml 0.7 ml
Retention and recovery 90% recovery of 20 bases or bp, 99% retention of salts 90% recovery of 20 bases or bp, 99% retention of salts 95% recovery of 22 bases or bp, 98% retention of ddNTPs 95% recovery of 22% bases or bp, 98% retention of ddNTPs
MW exclusion limit, globular proteins 6,000 6,000 40,000 40,000
Sample volume 50–100 µl 10–75 µl 50–100 µl 10–75 µl
Centrifuge type Swinging bucket Microcentrifuge Swinging bucket Microcentrifuge
Autoclavability Yes Yes Yes Yes


*150 mM NaCl, 17.5 mM sodium citrate, pH 7.0

Column Selection Guide
Column Selection Guide
  Bio-Spin 6 Micro Bio-Spin 6 Bio-Spin 30 Micro Bio-Spin 30 PCR Kleen
Packed support Special grade
Bio-Gel P-6 gel		 Special grade
Bio-Gel P-6 gel		 Special grade
Bio-Gel P-30 gel		 Special grade
Bio-Gel P-30 gel		 Special grade
Size exclusion gel
Equilibration buffer 10 mM Tris, pH 7.4 or SSC buffer* 10 mM Tris, pH 7.4 or SSC buffer* 10 mM Tris, pH 7.4 or SSC buffer* 10 mM Tris, pH 7.4 or SSC buffer* 10 mM Tris, 1 mM EDTA, pH 7.0
Applications Desalting of oligonucleotides >20 bases • • - - -
Labeling reactions:
removal of unincorporated nucleotides >20 bases or bp from DNA		 - - • • -
Removal of primers and primer-dimers from PCR products >200 bp - - - - •
Buffer exchange
(restriction fragments, PCR products, enzyme reactions, sequencing templates.)		 • • - • -
DNA sequencing
reaction mixture cleanup**		 - - • • -
Riboprobe cleanup*** - - - • -
Desalting of antibody, enzyme, and protein solutions • • - • -
Purification of proteins of moleular weight >6,000 • • - - -
Purification of proteins of molecular weight >40,000 - - • • -
Bed Volume 1.1 ml 0.7 ml 1.1 ml 0.7 ml 0.6 ml
Retention and recovery 90% recovery of 20 bases or bp, 99% retention of salts 90% recovery of 20 bases or bp, 99% retention of salts 95% recovery of 22 bases or bp, 98% retention of ddNTPs 95% recovery of 22 bases or bp, 98% retention of ddNTPs 85% recovery of ≥700bp, 95% retention of primers and primer-dimers
Molecular weight exclusion limit, globular proteins 6,000 6,000 40,000 40,000 8,000,000
Sample volume 50–100 µl 10–75 µl 50–100 µl 10–75 µl 25–100 µl
Centrifuge type Swinging bucket Microcentrifuge Swinging bucket Microcentrifuge Microcentrifuge
Autoclavability Yes Yes Yes Yes Yes

* 150 mM NaCl, 17.5 mM sodium citrate, pH 7.0
** In Tris buffer.
*** In RNase-free Tris buffer.

PCR and DNA Fragments
PCR Product and DNA Fragment Purification Guide

Bio-Rad offers a variety of pre-PCR and post-PCR sample preparation and cleanup columns and prepacked columns; many are also suitable for DNA fragment purification.

  Freeze 'N Squeeze™ DNA
Gel Extraction Spin Columns		 Micro Bio-Spin® and Bio-Spin Columns PCR Kleen™
Spin Columns
Application Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting Removal of PCR products >200 bp from other reaction mixture components
Starting material ≤700 mg gel slice 10–100 µl of sequencing reation mixture 25–100 µl of PCR or other enzyme reaction
Format Spin-column extraction of frozen-thawed gel slices Prepacked size exclusion spin column Prepacked size exclusion spin column
Recovery 4–23 kb: >80% recovery;
0.5–4 kb:>70% recovery;
50–500 bp:>50% recovery		 >95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators 50–80%
Time required <10 min <10 min <4 min


Bio-Spin 6 and Micro Bio-Spin 6 columns clean protein samples quickly using size exclusion chromatography. Filled with Bio-Gel P-6 gels, these columns are shipped fully hydrated in Tris buffer and are ready to use. They effectively clean and remove salts from protein samples in just 10 minutes.

Bio-Spin 6 and Micro Bio-Spin 6 columns:

  • Provide fast salt and contaminant removal in an easy-to-use spin-column format
  • Remove compounds <6 kD by size exclusion chromatography
  • Accommodate up to 100 µl of sample
Micro Bio-Spin 6 Columns

732-6221
Pkg of 25, prepacked spin columns with Bio-Gel P-6 in Tris buffer, sample volume 10–75 μl, 6,000 MW limit, includes 50 collection tubes

List Price:   $85.00
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Micro Bio-Spin 6 Columns

732-6222
Pkg of 100, prepacked spin columns with Bio-Gel P-6 in Tris buffer, sample volume 10–75 μl, 6,000 MW limit, includes 200 collection tubes

List Price:   $295.00
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Micro Bio-Spin 6 Columns

732-6225
Pkg of 1,000, prepacked spin columns with Bio-Gel P-6 in Tris buffer, sample volume 10–75 μl, ~6,000 MW limit, includes 2,000 collection tubes

List Price:   $2,365.00
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Bio-Spin 6 Columns

732-6227
Pkg of 25, prepacked spin columns with Bio-Gel P-6 gel in Tris buffer, sample volume 50–100 μl, 6,000 MW limit, includes 50 collection tubes

List Price:   $105.00
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Bio-Spin 6 Columns

732-6228
Pkg of 100, prepacked spin columns with Bio-Gel P-6 in Tris buffer, sample volume 50–100 μl, 6,000 MW limit, includes 200 collection tubes

List Price:   $365.00
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Number Description Options
4006051 Instruction Manual, Micro Bio-Spin Chromatography Columns, Rev B Click to download
4106140 Instruction Manual, Micro Bio-Spin Columns P-30 Tris, RNase Free, Rev C Click to download
LIT507 Instruction Manual, Bio-Spin 6 and 30 Columns, Rev G Click to download [ Add to Cart ]
4110104 Instruction Manual, Bio-Spin 30 Tris Columns, Rev C Click to download
2320 Bio-Spin and Micro Bio-Spin Column Product Information Sheet, Rev A Click to download