| |
STEM Electrophoresis
Classroom Kit
166-5090EDU |
STEM Electrophoresis
Teacher Demonstration Kit
166-5080EDU |
| Kit Contents (sufficient materials for 8 student workstations with 2–4 students per workstation) |
| Store at room temperature |
| Dye extraction solution, 25 ml |
1 |
1 |
| Blue 1 reference dye, 150 µl |
1 |
1 |
| Yellow 5 reference dye, 150 µl |
1 |
1 |
| Yellow 6 reference dye, 150 µl |
1 |
1 |
| Red 40 reference dye, 150 µl |
1 |
1 |
| Electrophoresis buffer, 50x TAE, 100 ml |
1 |
1 |
| Molecular biology grade agarose, 5 g |
1 |
1 |
| Microcentrifuge tubes, 2 ml |
72 |
72 |
| Hinged plastic boxes |
4 |
1 |
| Paper clips |
16 |
4 |
| Black alligator clip leads |
8 |
2 |
| Red alligator clip leads |
8 |
2 |
| 8-well combs |
8 |
2 |
| Curriculum (includes teacher guide, student manual, and graphic quick guide) |
1 |
1 |
| Required Accessories Not Included in Kit |
| 9 V batteries (3–5 per workstation) |
24–40 |
6–10 |
| Plastic rulers or plastic cards to cut gels |
8 |
2 |
| 2–20 µl adjustable-volume micropipets or 10 µl fixed-volume micropipets |
8 |
2 |
| 2–200 µl pipet tips, 1,000/bag |
1 |
1 |
Eyedroppers or
100–1,000 µl adjustable-volume micropipets
or disposable plastic transfer pipets (DPTPs) |
8
40 |
2
10 |
| 100–1,000 µl pipet tips* |
1 bag |
1 bag |
| Permanent markers |
8 |
2 |
| Plastic cups or small beakers |
32 |
8 |
| Microwave oven or hot plate |
1 |
1 |
| Distilled water |
3 liters |
1 liter |
| 500 ml Erlenmeyer flask for microwaving agarose |
1 |
1 |
| Candies with a variety of color coatings |
Variable |
Variable |
| Balance |
1 |
1 |
| Recommended (Optional) Accessories |
| Microcentrifuge or mini centrifuge |
1 |
1 |
| Digital camera for imaging gels |
1 |
1 |
| Microcentrifuge tube rack |
8 |
1–2 |
| * Not needed if using DPTPs or eyedroppers instead of 100–1,000 µl adjustable-volume micropipets. |
