T100™ Thermal Cycler

The 5.7'' high-resolution touch screen makes it easy to create a protocol, start a run, or manage files, so you spend less time programming.

• Save time with streamlined graphical programming
• Stay organized with personalized folders
• Effortlessly transfer protocols among instruments using a USB flash drive
• Get started quicker with the built-in library of standard protocols for long PCR, fast PCR, reverse transcription PCR, and more

Home screen.		New Protocol screen.

Optimize Reactions in a Single Run

Thermal gradient technology allows you to quickly optimize your PCR assays in a single run by simultaneously testing eight different temperatures across a range of up to 25°C. The optimal annealing temperature for a PCR assay maximizes yield while preventing the formation of nonspecific products. The dynamic ramping technology adjusts the cycler ramp rate, ensuring that the incubation times across the eight rows during a gradient step are identical.

Optimization of an assay results in better yields and specificity. Results show that assays A and B can be run at an annealing temperature of 57.9°C on the same plate. Higher temperatures result in a reduced yield in assay A while lower temperatures result in nonspecific products in assay B. M, markers.