Crude Genomic DNA for PCR: InstaGene Matrix
InstaGene matrix, composed of specially formulated Chelex resin, makes DNA sample preparation fast, easy, and cost-effective, providing PCR-quality template DNA from crude genomic preps in less than 1 hour. The InstaGene matrix adsorbs cell lysis products and removes inhibitors such as magnesium ions that interfere with the PCR amplification process. Simply place cells in a microcentrifuge tube, add the InstaGene matrix, boil, and spin. PCR-ready DNA is in the supernatant and can be transferred directly into PCR reaction tubes
Total RNA Isolation: Aurum Total RNA Mini Kit
The Aurum total RNA mini kit produces DNA-free total RNA from a wide range of starting materials, including cultured cells, bacteria, and yeast, as well as animal and plant tissues. The Aurum total RNA mini kit can also be used for RNA cleanup and desalting.
Quantum Prep Plasmid Miniprep Kit
Obtain optimal yield of plasmids for gene transfer, restriction, ligation, and cloning protocols. The Quantum Prep miniprep kit is tested to ensure at least 20 µg of high-copy plasmid yield from just 1.5 ml of bacterial culture. The Quantum Prep miniprep procedure takes less than 15 minutes from cell culture to purified plasmid, using an easy spin column-based procedure. DNA is recovered in water or Tris-EDTA buffer for immediate use in mapping, sequencing, transfection, transformation, PCR, or subcloning.
PCR Kleen Spin Purification Columns
Try our prepacked spin columns for purifying PCR products and other DNA molecules >200 bp directly from reaction mixtures. A simple 4 minute spin effectively removes salts, nucleotides, enzymes, primers, and primer-dimers. Purified DNA fragments are eluted into a collection tube and are immediately available for secondary PCR, subcloning, restriction digests, ligations, sequencing, and other enzymatic manipulations.
Aurum Plasmid Mini Purification KitOur easy-to-use Aurum Plasmid Mini kit improves the efficiency and throughput of plasmid purifications with a simple bind-wash-elute protocol using silica membranes. Lysed bacterial cultures can be cleared either by centrifugation or by vacuum filtration with the Aurum Vacuum Manifold. The clarified lysates are then applied to plasmid binding columns and plates where DNA is bound, then washed and finally eluted — all in less than 10 minutes. The purified plasmid DNA can be immediately used in any downstream molecular biology application. For more information, request bulletin 2664.
