Maximize Reaction Efficiency and Increase Accuracy of Quantitation
Selecting the optimal annealing temperature for your oligo reduces amplification of nonspecific products. The thermal gradient feature of the MJ Mini cycler with the MiniOpticon system makes finding the optimal temperature easy, by allowing you to program a temperature gradient across the sample block for any incubation step. After analysis of PCR products, use the gradient calculator to see which temperature yielded the best results. Simply enter the temperature range, and the calculator displays the exact temperature at which each sample was run. With thermal gradient technology, your PCR reactions can be optimized in a single experiment.
For any incubation step in your PCR protocol, you can simultaneously incubate samples at up to eight temperatures with a range of 1–16°C. This makes finding the appropriate temperature easy and allows you to optimize your PCR reactions in a single experiment.
