Individual Autoantibody EIA Profiling

Bio-Rad offers a full range of individual autoantibody EIA assays by means of two well-established product families: Bio-Rad Autoimmune EIA and Kallestad™ EIA. Both product families feature common assay protocols, common reagents, color-coded breakaway wells and easy automation, allowing for simple, objective and sensitive autoimmune diagnostics. These assays can be used in conjunction with ANA and ENA assays to identify specific autoantibodies associated with autoimmune diseases.

Bio-Rad Autoimmune EIA Assay Characteristics

• Sample dilution: 1:40 (only Anti-dsDNA and Anti-ssDNA are 1:100)
• Sample volume: 10 µL
• Incubation time: 90 minutes (30/30/30)
• Incubation temperature: Room temperature (18–27°C)
• Conjugate: HRP
• Substrate: TMB

Kallestad EIA Assay Characteristics

• Sample dilution: 1:100
• Sample volume: 10 µL
• Incubation time: 120 minutes (60/30/30)
• Incubation temperature: Room temperature (18–25°C)
• Conjugate: Alkaline phosphatase
• Substrate: Phenolphthaleinmonophosphate (PMP)