GS HIV-1/HIV-2 PLUS O EIA

GS HIV-1/HIV-2 PLUS O EIA

True Detection of HIV-1 Group O

The only HIV-1/HIV-2 microplate assay approved for diagnostic and blood screening use to test human serum, plasma, and cadaveric serum samples.

Detects antibodies to HIV-1 (groups M and O) Detects antibodies to HIV-2		For manual and automated use		Common reagents can be used with other Bio-Rad EIA assays
Color changes during protocol steps		Offers results in 2.5 hours		Microplate strip identification

Ensuring Detection of All HIV Groups and Variants

Reactivity in HIV-1 (Groups M and O) and HIV-2 Positive Samples
Results obtained with GS HIV-1/HIV-2 PLUS O EIA

* Included 313 AIDS patients, 490 known HIV-1 positive samples from the U.S., and 199 known HIV-1 positive samples from non-U.S.
   countries. [Australia, New South Wales (N = 36), Central African Republic (N = 40), Ghana (N = 5), Kenya (N = 3), Nigeria (N = 46),
   Sierra Leone (N = 40), Thailand (N = 21), Zimbabwe (N = 8)].
† HIV-2 samples were repeatedly reactive on an HIV-2 EIA, positive on an HIV-2 Western blot, and indeterminate or negative on an
HIV-1 Western blot.
‡ Samples were characterized as HIV-1 group O by serotype and/or genotype.

Closing the Serological Window

Reactivity on 50 HIV-1 Seroconversion Panels
Results Obtained with GS HIV-1/HIV-2 PLUS O EIA

* Four of the 50 seroconversion panels did not have test results with the licensed HIV-1/HIV-2 EIA Kit # 1 and are no longer available
   for testing.

GS HIV-1/HIV-2 PLUS O EIA

Test Format			Sample/Reagent Addition Monitoring
			Color of well BEFORE addition		Color of well AFTER addition
		Coated microplate* 25 mL of Specimen Diluent		clear to purple
		75 µL of control or sample Incubate 60 minutes at 37°C		purple to blue
Wash
		100 µL of Working Conjugate Solution Incubate 30 minutes at 37°C		clear to green
Wash
		100 µL of Working TMB Solution Incubate 30 minutes at room temperature		clear to blue***
		100 µL of Stopping Solution		blue to yellow***
READ within 30 minutes at 450 nm with the 615–630 nm filter as a reference
* Microplate coated with: HIV-1 gp160 rDNA protein. HIV-1 p24 rDNA protein. Synthetic polypeptide mimicking artificial HIV-1 group O epitope. HIV-2 gp 36 polypeptide. ** Conjugate (peroxidase-conjugated antigens): Two HIV-1 gp41 polypeptides. HIV-1 p24 rDNA protein. Synthetic polypeptide mimicking artificial group O epitope. HIV-2 gp36 polypeptide. *** Reactive samples after 30 minute incubation.

HIV Serological Profile