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Lambda DNA gel has been a workhorse of molecular biology for decades. It is vital in the fields of molecular cloning and genomic sequencing since it can be used to subclone very long genomic DNA fragments much more efficiently than plasmid vectors. Lambda DNA comes from a bacterial virus, or bacteriophage, which attacks bacteria by injecting them with its nucleic acid. Once inside, Lambda DNA hijacks the bacterial cellular machinery and replicates itself until the cells burst, releasing millions more bacteriophage to carry out the same infection process. Bacteriophage lambda is harmless to humans and other eukaroytic organisms, and therefore makes an excellent source of DNA for experimental study.
The lambda genome has approximately 48,000 base pairs. Each restriction enzyme used in this kit will cut the lambda DNA several times, generating distinct sets of DNA restriction fragments of different sizes. The three different sets of DNA fragments that result are separated by agarose gel electrophoresis and visualized using Bio-Rad's safe Fast Blast DNA stain.
Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use three different restriction enzymes to digest genomic DNA from lambda bacteriophage. By visualizing the effects of three different enzymes on identical samples of double-stranded DNA, students learn that different restriction enzymes recognize and cut different DNA sequences.
Features and Benefits
With this kit, students are able to:
Students learn the procedures and principles of restriction digestions and DNA gel electrophoresis, including:
How It Works
The lambda genome has approximately 48,000 base pairs. Each restriction enzyme used in this kit cuts the lambda DNA several times, generating distinct sets of DNA restriction fragments of different sizes. The three different sets of DNA fragments that result from the enzymatic digestion are separated by agarose gel electrophoresis and visualized using Bio-Rad's safe Fast Blast™ DNA stain. Banding patterns from each sample are then compared to each other and to a DNA size standard. Students use their electrophoresis results to construct standard curves and determine the precise DNA fragment sizes generated by the different restriction enzymes.
More Information
166-0002EDU DNA restriction analysis classroom study kit, includes DNA, restriction enzymes, buffers, agarose, Fast Blast DNA stain, curriculum, and more, for 32 students; education use only
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