Criterion™ TGX™ Precast Gels

Criterion™ TGX™ Precast Gels

Criterion TGX precast gels are based on the long shelf life TGX formulation. The gels deliver superior resolution, fast run times, and greater transfer efficiencies.

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Ordering Information

Overview

The Criterion™ TGX™ (Tris-Glycine eXtended) precast gels for PAGE are based on the long shelf life TGX formulation. The TGX gels retain Laemmli-like separation characteristics using the standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 min at 300 V while maintaining low temperatures.

Benefits Include:

  • Run time as short as 20 min
  • Protein transfer in as little as 15 min
  • Direct drop-in for Laemmli systems
  • Inexpensive Laemmli buffer system, low running costs
  • High-throughput system

TGX gels are manufactured without SDS. The gels can be used to run proteins under nondenaturing conditions for subsequent analysis of their native conformation and activity by omitting SDS from the running buffer. Native analysis does not provide accurate molecular weight information. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.

Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion™ Dodeca™ cell.

Specifications

Gel dimensions (Width x Length x Thickness)
133 x 87 x 1 mm
Cassette dimensions (Width x Length x Thickness)
150 x 106 x 5.3 mm
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Gel storage conditions
Store flat at 4°C; do not freeze
Shelf life at 2-8°C*
12 months
Recommended sample buffer (Laemmli, dilute 1:1 with sample)
62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/glycine/SDS)
25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
Run times at constant voltage: 200 V
42–50 min
Run times at constant voltage: 300 V
20–26 min

Ordering

items

Use the filters below to refine results!

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5671023
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671024
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671025
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671033
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671034
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671035
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671071
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671081

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671082

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671083

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671084

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671085

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671091

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671092

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671093

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671094

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671095

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671101
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671102
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671103
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671104
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671105
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671111
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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5671121

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671122

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671123

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671124

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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5671125

Pkg of 1, AnykD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Accessories

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(Discontinued) Criterion™ Cell
1656001
Vertical midi-format electrophoresis cell, includes buffer tank, lid with power cables, 3 sample loading guides (12+2-well, 18-well, and 26-well)
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1654130
Vertical midi-format electrophoresis cell, includes buffer tank with built-in cooling coil, lid with power cables, 12 gel capacity
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(Discontinued) Gel Releasers
1653320

Pkg of 5, gel releaser for Mini-PROTEAN® Tetra system, Mini-PROTEAN® 3 Dodeca™ cell, Mini-PROTEAN 3 multi-casting chamber, and PROTEAN® Plus Dodeca™ cell

Kits & Reagents

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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

Documents

Number Description Options
6507
TGX and TGX Stain-Free Gels: Publications List
6040
Electrophoresis Guide, Interactive PDF, Rev C
5981
Criterion Tris-HCl to Criterion TGX and TGX Stain-Free Precast Gels Catalog Number Conversion Chart
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H
6493
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
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