ChemiDoc Imaging System

ChemiDoc Imaging System

Use the ChemiDoc Imaging System for rapid, sensitive detection of chemiluminescence blots, a wide range of stains, and gel documentation.

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Overview

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Stain Free Enabled

What is Stain-Free Technology?

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The ChemiDoc Imaging System provides fast, reliable, and sensitive imaging and documentation of gels and chemiluminescence western blots. This system is compatible with stain-free technology, chemiluminescence detection, and a wide range of gel stains such as ethidium bromide, SYPRO Ruby, Coomassie, and silver stains.

The ChemiDoc Imaging System can be upgraded to gain the full fluorescent western blotting capabilities of the ChemiDoc MP System. The upgrade package has an increased spectrum (RGB, far red, and near infrared), allowing multiplex fluorescence western blotting capability with minimal cross-talk between channels. Multiplex fluorescence allows detection of multiple antigens on the same blot, eliminating the need for stripping and reprobing.

Stain-Free Western Blotting provides efficient, consistent electrophoresis and blotting with the ability to rapidly assess electrophoresis and blot transfer quality, and perform total protein normalization.

ChemiDoc Imaging Systems

Complete Capabilities: Superior Images

Stain-Free
Total Protein Normalization

 

Learn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
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Chemiluminescence
Replaces Film

 

Save money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
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Multiplex Fluorescence
3 Colors

 

Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
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Features and Benefits

  • All-in-one flexible imaging — get precise, reproducible chemiluminescence and colorimetric blot detection, analysis, and gel documentation in a single system
  • Sample Trays and Smart Tray Technology — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
  • Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminate the need for housekeeping proteins
  • Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
  • Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.

Applications and Uses

  • Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
  • Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with chemiluminescence, or colorimetric detection

Image Lab Touch Software Demo

Explore how intuitive it is to acquire fluorescent and chemiluminescent gels and blots on the ChemiDoc systems. This easy-to-use software is perfect for multi-group use and comes installed on both the ChemiDoc and ChemiDoc MP.

Learn More about Image Lab Touch Software

Included Components

  • Internal computer with Image Lab Touch Software
  • 12” touch screen display
  • Image Lab Software to install on your computer
  • Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)

Mini and Midi Gel Stain-Free Western Blotting include:

  • ChemiDoc Imaging System
  • Mini-PROTEAN Tetra Cell, 4-gel (1658004) or Criterion Cell (1656001) Electrophoresis Chamber
  • PowerPac Basic Power Supply (1645050)
  • Trans-Blot Turbo Transfer System (1704150)
  • 4–20% Mini-PROTEAN TGX Stain-Free or 4–20% Criterion TGX Stain-Free Protein Gels
  • Buffers, Nitrocellulose Transfer Packs, Immun-Blot LF PVDF Membranes, and other consumables

Optional Components

  • White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR Stain (12003027)
  • Holder for ChemiDoc Sample Trays and UV Shield (1708377)
  • Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
  • UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)

Configuration Options

Product Catalog Number
ChemiDoc Imaging System 12003153
ChemiDoc to ChemiDoc MP Upgrade Kit 12003263

Note: Also available for increased capabilities are the ChemiDoc MP Imaging System (12003154), which provides a broader spectrum (RGB, far red, and near infrared), multiplex fluorescence western blotting, and reduced crosstalk.

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Specifications

Automation Capabilities
 
Smart Tray Technology™
Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Autofocus
Precalibrated focus for any zoom setting or sample height
Auto-exposure
Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding
Dynamic; precalibrated and optimized for each application
Hardware
 
Touch-screen functionality
Multitouch capability (2 points) 12.1" display
Maximum image area (W x H)
21 x 16.8 cm
Detector
Cooled CCD, 6 megapixels
Dynamic range
>4 orders of magnitude
Illumination sources
Trans-UV, 302 nm excitation
Epi-white
Trans-white (requires White Sample Tray)
Trans-blue, 450–490 nm excitation (requires Blue Sample Tray)
Detection
Chemiluminescence filter
590/110 nm standard emission filter for protein and DNA gel and blot imaging
Data output
16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight
35 kg (78 lb)
Instrument size (L x W x H)
61 x 51 x 53 cm
Operating voltage
100–250 V
Operating temperature
10–28°C
Operating humidity
10–85% relative humidity (non-condensing)

Ordering

items

Use the filters below to refine results!

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12003153

The ChemiDoc Imaging System allows sensitive imaging and documentation of DNA/protein gels and chemiluminescent western blots. It can perform a wide range of blotting applications including Stain-Free technology, ethidium bromide, SYPRO Ruby, Coomassie, and silver stains. Includes internal computer, 12” touch-screen display, camera, Blot/UV/Stain-Free Sample Tray, Image Lab™ Touch Software, and Image Lab Software.

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12003263
Pkg of 1, includes blue, green, red, far red, near infrared illumination sources and emission filters
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Image Lab Touch Software on USB drive
12014300

USB flash drive containing Image Lab Touch Software version 3.0.1 upgrade for use with Bio-Rad ChemiDoc and ChemiDoc MP Imaging Systems. Find release notes​ and installation instructions here.

Accessories

Image
12003026-white-sample-tray-chemidoc-mp-chemidoc-imaging-systems_thumb.jpg
12003026
Pkg of 1, sample tray for gels and blots stained with Coomassie Blue, copper, silver, or zinc stains
Image
12003027-blue-sample-tray-chemidoc-mp-chemidoc-imaging-sytems_thumb.jpg
12003027
Pkg of 1, sample tray for nucleic acid applications with GelGreen or any SYBR® Stain
Image
12003028-blot-uv-stain-free-sample-tray-chemidoc-mp-chemidoc-imaging-systems_thumb.jpg
12003028
Pkg of 1, sample tray for imaging chemiluminescence and fluorescence blots, stain-free gels and blots, and gels stained with ethidium bromide, SYPRO Ruby, Oriole, or SYBR® Stains
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12005137-tn.jpg
12005137
This kit ensures your ChemiDoc and ChemiDoc MP Instruments are properly installed and calibrated according to the technical specifications for optimal performance according to regulatory guidelines
Image
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12003915
Pkg of 2, alignment templates for use with ChemiDoc MP and ChemiDoc Imaging Systems

Documents

Number Description Options
3429
The Complete Guide to Stain-Free Western Blotting
6356
Traditional vs. Stain-Free Western Blotting
6253
Stain-Free Western Blotting Brochure
[ Add to Cart (Free) ]
7051
Imaging Systems Family Flier
[ Add to Cart (Free) ]
6967
ChemiDoc Imaging System Flier
6390
General Stain-Free Western Blotting Protocol
[ Add to Cart (Free) ]
10000062126
ChemiDoc and ChemiDoc MP Imaging Systems with Image Lab Touch Software User Guide Version 3.0.1 (English)
100000131171
ChemiDoc and ChemiDoc MP Imaging Systems with Image Lab Touch Software Use Guide Version 3.0.1 (Simplified Chinese)
6427
Bio-Rad Stain-Free Western Blotting
[ Add to Cart (Free) ]
10000060300
Acquiring an Image with ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver A
10000060301
Acquiring a Multichannel Image with the ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver B
6493
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
[ Add to Cart (Free) ]