CHEF Mapper XA System

CHEF Mapper XA System

The CHEF Mapper XA system incorporates patented FIGE and AFIGE technologies for superior resolution from 100 bp to 10 Mb, in addition to the features of the CHEF-DR II and the CHEF-DR III systems.

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Overview

The CHEF Mapper XA system incorporates patented FIGE and AFIGE* technologies for superior resolution in the range of 100 bp to 10 Mb, in addition to the features of the CHEF-DR II and the CHEF-DR III systems. The CHEF Mapper XA system also includes algorithms for deriving separation conditions. This system is ideal for both the PFGE novice and the seasoned expert.

Each CHEF Mapper XA system is supplied with a power module, embedded auto-algorithm for protocol optimization, interactive algorithm program disk, electrophoresis cell, cooling module, variable-speed pump, and accessory kit.

Automation

The CHEF Mapper XA system offers two ways to optimize your separations. The unique auto-algorithm can automatically select optimal separation conditions, integrating 11 key variables and implementing starting separation conditions. Protocols can be refined using the Windows operating system-based interactive algorithm, which lets you specify several run variables simultaneously to derive optimal separation protocols.

Customization

Busy laboratories need equipment that can store and readily access key separation conditions. The CHEF Mapper XA system can store up to 99 programs.

Application Versatility

The CHEF Mapper XA system is the most flexible of the PFGE units. The CHEF Mapper XA system achieves higher resolution with greater speed and accuracy than any other PFGE system, making it ideal for all PFGE applications. The CHEF Mapper XA system lets you choose any pulse angle from 0 to 360°, which allows optimal separation of both chromosomal and plasmid DNA with one system. Accurate sizing of fragments requires an expanded linear range of separation. Switch-time ramps increase the mobility of fragments by gradually changing the switch times during the course of a run. Nonlinear (for example, concave or convex) ramps change the switch-time increments from the start to the end of a run. These nonlinear ramps separate fragments linearly from 50 to 700 kb, yielding more precise fragment size measurements. The multistate mode of the CHEF Mapper XA system enhances resolution in selected fragment size ranges. Each vector (angle of pulse) can be assigned its own voltage (field intensity) and its own switch time (duration of pulse). Up to eight different states can be combined into one run to optimize the separation of subsets of fragments in the sample. The application of secondary pulses of defined voltage, duration, angle, and frequency can enhance the separation and resolution of very large DNA molecules by releasing DNA caught in the gel matrix.

* U.S. patent 5,549,796.

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Specifications

Power Module
 
Dimensions (W x D x H)
55.9 x 34.5 x 30 cm
Construction
Aluminum chassis
Weight
16 kg (35.3 lb)
Power supply
350 V maximum, to allow maximum gradient of 9 V/cm, continuously adjustable; built in
Maximum current
0.5 A
Allowable voltage gradients
0.6–9 V/cm, in 0.1 V/cm increments
Battery back-up
All parameters in memory, up to 3 hr of interruption
Delayed start
Up to 72 hr
Electrode potentials
Dynamically regulated (feedback adjustment) +/- 0.5% F.S.
Program storage
20 average size protocols
Display
Fluorescent, 2 lines x 40 characters per line, alphanumeric
Environmental
 
Operating temperature
50°F (10°C) to 90°F (32°C); 30–80% humidity
Storage temperature
32°F (0°C) to 140°F (60°C); 10–90% humidity
Switching Functions
 
Switching range
50 ms to 18 hr
Switch angle variable
0–360° (all electronic switching) in 0.5° increments
Multistate vector switching
Up to 15 vectors per pulse cycle, each definable by angle, voltage, and duration
Switch time ramps
Linear, concave, or convex using hyperbolic function
Secondary pulses
Defined by voltage, frequency, angle, and duration
Field inversion (FIGE)
Available with asymmetric forward, reverse voltages
Maximum program blocks
8, with automatic execution
Maximum run time
999 hr per block
Fuses
3 A Slow-Blow; two each for AC line input
Electrophoresis Cell
 
Dimensions (W x D x H)
44.2 x 50.3 x 11.4 cm, horizontal format
Construction
Cover: vacuum-formed polycarbonate, base: injection-molded polycarbonate
Lid
Safety interlocked
Weight
10.2 kg (22.5 lb)
Electrodes
24, platinum (0.02" diameter)
Temperature monitoring
Via precision temperature probe mounted in base of cell
Accessories Included
 
Variable speed oscillating pump
120 V, ground isolated; flow rate 1 liter/min, typical
Casting stand
14 x 13 cm
Comb
15-well comb and comb holder
Tygon tubing
365 cm
Disposable sample plug mold
50 slot
Yeast DNA standard
S. cerevisiae YNN295
Chromosomal grade agarose
5 g
Pulsed field Certified™ agarose
5 g
Leveling bubble
1
Cooling Module (optional)
 
Weight
14 kg (30.9 lb)
Construction
Aluminum
Dimensions (W x L x H)
23 x 42 x 24 cm
Cooling capacity
75 W of input power at 14°C
Operating range
5–25°C
Total system weight
41.7 kg (91.9 lb)

Ordering

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(Discontinued) CHEF Mapper XA Chiller System, 120 V
1703670
120 V, complete PFGE system, includes cooling module, pump, tubing, S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose
170-3670_tn.jpg
(Discontinued) CHEF Mapper XA Chiller System, 220 V
1703672
220 V, complete PFGE system, with cooling module, icludes pump, tubing, S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

Accessories

chef electrophoresis cell
(Discontinued) CHEF Electrophoresis Cell
1703649

Horizontal electrophoresis cell for use with with CHEF Mapper XA, CHEF DR-II, and CHEF DR-III Variable Angle systems

170-3688_tn.jpg
(Discontinued) CHEF Cooling Module, 100 V
1703688
pulsed field gel electrophoresis cooling system, for use with all 100 V CHEF chiller systems
170-3655_tn.jpg
(Discontinued) CHEF Cooling Module, 220/240 V
1703655
pulsed field gel electrophoresis cooling system, for use with all 220/240 V CHEF chiller systems
170-3654_tn.jpg
(Discontinued) CHEF Cooling Module, 120 V
1703654
pulsed field gel electrophoresis cooling system, for use with all 120 V CHEF chiller systems
170-3689_tn.jpg
(Discontinued) CHEF Standard Casting Stand
1703689
Pkg of 1, pulsed field gel electrophoresis casting stand, includes 14 x 13 cm frame and platform, for use with any CHEF system
170-3704_tn.jpg
(Discontinued) CHEF Wide/Long Combination Casting Stand
1703704
Pkg of 1, pulsed field gel electrophoresis casting stand, includes 21 x 14 cm frame and platform, for use with any CHEF system

Kits & Reagents

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(Discontinued) Certified Megabase Agarose, 500 g
1613110
500 g, agarose for use with CHEF and FIGE applications, certified free of inhibitors, DNases, RNases
161-3100_tn.jpg
(Discontinued) Certified Molecular Biology Agarose, 25 g
1613100
25 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
161-3101_tn.jpg
(Discontinued) Certified Molecular Biology Agarose, 125 g
1613101
125 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
161-3102_tn.jpg
(Discontinued) Certified Molecular Biology Agarose, 500g
1613102
500 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases
162-0137_tn.jpg
(Discontinued) Pulsed Field Certified Agarose, 100 g
1620137
100 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases
162-0138_tn.jpg
(Discontinued) Pulsed Field Certified Agarose, 500 g
1620138
500 g, agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, RNases

Combs

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(Discontinued) 20-Well Comb, Adjustable Height, 1.5 mm Thickness
1704322

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

170-4344_tn.jpg
(Discontinued) 30-Well Comb, Adjustable Height, 1.5 mm Thickness
1704344

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

170-3627_tn.jpg
(Discontinued) 15-Well Comb, 21 cm x 1.5 mm
1703627
Pkg of 1, 15-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
170-3628_tn.jpg
(Discontinued) 30-Well Comb, 21 cm x 1.5 mm
1703628
Pkg of 1, 30-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
170-3645_tn.jpg
(Discontinued) 45-Well Comb, 21 cm x 1.5 mm
1703645
Pkg of 1, 45-well pulsed field gel electrophoresis comb, for use with CHEF-DR® and CHEF Mapper® Systems (requires comb holder #1703699) or any CHEF system
170-4326_tn.jpg
(Discontinued) 10-Well Comb, Adjustable Height, 1.5 mm Thickness
1704326

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

170-4325_tn.jpg
(Discontinued) 10-Well Comb, Adjustable Height, 0.75 mm Thickness
1704325

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

170-4324_tn.jpg
(Discontinued) 15-Well Comb, Adjustable Height, 1.5 mm Thickness
1704324

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

170-4323_tn.jpg
(Discontinued) 15-Well Comb, Adjustable Height, 0.75 mm Thickness
1704323

For use with Sub-Cell and Wide Mini-Sub Cell GT Systems (requires comb holder #1704320) or CHEF-DR and CHEF Mapper Systems (requires comb holder #1703699)

Standards

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(Discontinued) CHEF DNA Size Marker, 0.2–2.2 Mb, S. cerevisiae Ladder
1703605
5 agarose blocks, PFGE marker, 0.225–2.2 Mb S. cerevisiae chromosomal DNA, for 25–40 plugs
170-3624_tn.jpg
(Discontinued) CHEF DNA Size Standard, 4.9–98 kb Ladder
1703624
200 μl, 0.1 μg/μl, PFGE standard, 5 kb ladder, 4.9–120 kb, concatemers of pBR328, 20–25 applications
170-3707_tn.jpg
(Discontinued) CHEF DNA Size Standard, 8.3–48.5 kb, Lambda Ladder
1703707
125 μl, 0.2 μg/μl, PFGE standard, 8.3–48.5 kb, mixed digests of phage lambda (λ), 125 applications
170-3635_tn.jpg
(Discontinued) CHEF DNA Size Standard, 48.5–1,000 kb, Lambda Ladder
1703635
5 agarose blocks, PFGE standard, lambda (λ) ladder, 48.5-1000 kb in 48.5 kb increments, concatemers of λcl857Sam7 lambda DNA per block, for 25–40 plugs

Documents

Number Description Options
M1703650
Instruction Manual, CHEF Mapper® XA, Rev E
5901
Pulsed Field Gel Electrophoresis Brochure, Rev B
1906
CHEF Mapper Pulsed Field Electrophoresis System Product Information Sheet
1637
CHEF Mapper™ and CHEF Mapper XA System Specifications
4006187
Instruction Manual, CHEF Mapper ROM Upgrade Kit (Y2K), Rev A
4000066
Instruction Manual, Cooling Module
1659
Extending the Upper Limits of Pulsed Field DNA Electrophoresis Using Programmed Voltage Gradients Bulletin
1753
Preparation and Restriction Digestion of Escherichia coli Chromosomal DNA in Agarose Plugs for Use in PFGE Bulletin, Rev A
1589
Preparation and Separation of Mammalian DNA by Pulsed Field Gel Electrophoresis Bulletin, Rev A
1624
Model 1000 Mini Chiller Product Information Sheet
9204213
Instruction Manual, CHEF Variable Speed Pump, Rev B
LIT526
Instruction Manual, Standard Casting Stand Combination Comb Holder Wide/Long Combination Casting Stand
6228
Using Pulsed Field Gel Electrophoresis Standards, Rev A
6226
Pulsed Field Gel Electrophoresis Protocol