More Real-Time PCR Data from a Single Reverse Transcription Reaction
Hercules, CA — August 15, 2011 — Bio-Rad Laboratories, Inc.'s new iScript Advanced cDNA synthesis kit for RT-qPCR allows researchers to generate more real-time PCR data from a single 20 µl reverse transcription reaction for their gene expression analyses.
With a maximum capacity of 7.5 µg of input RNA, the iScript Advanced cDNA synthesis kit for RT-qPCR surpasses the capacity of its nearest rival by two micrograms. The ability to use more RNA in a reaction means researchers can:
- Improve reproducibility (a single RT reaction is diluted for qPCR analysis of all target genes, including housekeeping genes)
- Reduce cost per qPCR data point (several-fold higher RNA input with minimal increase of RT cost per reaction)
- Increase sensitivity for certain target genes (low copy targets can be detected within standard number of qPCR cycles)
The iScript Advanced cDNA synthesis kit for RT-qPCR offers additional advantages over competitor kits in terms of simplicity and speed. The kit has only two essential components: an iScript Advanced reaction mix and an iScript Advanced reverse transcriptase. The protocol takes only 35 minutes. By contrast, competitor cDNA synthesis kits contain up to seven components, and some "two-tube kits" recommend an additional optional step, resulting in a protocol that requires an hour or more to complete.
The iScript Advanced cDNA synthesis kit for RT-qPCR offers excellent sensitivity and efficiency in both real-time PCR (using SYBR® Green, EvaGreen, or probes) and conventional RT-PCR.
The iScript Advanced cDNA synthesis kit is part of the iScript family of products formulated for efficient and sensitive reverse transcription over a wide linear dynamic range of input RNA for real-time qPCR. Available products include:
- The original two-tube iScript cDNA synthesis kit for reverse transcription of standard total RNA inputs between 1 µg and 1 pg
- The iScript reverse transcription supermix, a streamlined (1 tube) version for improved reproducibility and high-throughput applications
- The iScript Select kit, which enables a choice of priming strategies, including gene-specific priming, for standard total RNA inputs between 1 µg and 1 pg
For more information on the iScript family, visit www.bio-rad.com/iScript.
About Bio-Rad
Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb), has remained at the center of scientific discovery for more than 50 years, manufacturing and distributing a broad range of products for the life science research and clinical diagnostic markets. The Company is renowned worldwide among hospitals, universities, major research institutions, as well as biotechnology and pharmaceutical companies, for its commitment to quality and customer service. Founded in 1952, Bio-Rad is headquartered in Hercules, California, and serves more than 100,000 research and industry customers worldwide through its global network of operations. The Company employs over 6,800 people globally and had revenues exceeding $1.9 billion in 2010. For more information, visit www.bio-rad.com.
For more information contact:
Viresh Patel
Bio-Rad Laboratories, Inc.
800-876-3425 ext 3926
Viresh_Patel@bio-rad.com
Ken Li
Chempetitive Group
312-997-2436 ext 109
kli@chempetitive.com