Precision Plus Protein™ WesternC™ Standards

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Informazioni Generali

Precision Plus Protein™ WesternC™ Standards

Gel (left), chemiluminescent detection (middle), fluorescent detection (right).

Precision Plus WesternC Graph

Standard curve generated by plotting log molecular weight (MW) versus migration distance (Rf) of each band in Precision Plus Protein Kaleidoscope standards through an SDS-PAGE gel.

Precision Plus Protein™ standards for protein electrophoresis offer unsurpassed band sharpness, accurate MW determination, lot-to-lot consistency, and excellent blotting results. These standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD and include three high-intensity reference bands (25, 50, and 75 kD).

Precision Plus Protein™ WesternC™ standards are prestained, broad-range protein standards that enable chemiluminescent detection when probed with StrepTactin-HRP conjugates, so the protein standard appears directly on a film or CCD image.

Key Features

  • Clean, sharp bands with MW confirmed by mass spectrometry
  • Highly accurate MW estimation, r2 values >0.99
  • Proprietary staining technology that provides batch-to-batch MW consistency and reproducible electrophoretic migration
  • Dual-color prestained bands (reference bands prestained pink, all others prestained blue)
  • Prestained bands for monitoring of electrophoresis progression and assessment of blotting efficiency
  • Strep-tag affinity peptide for detection and MW determination on western blots
  • Supplied as ready-to-use solution
  • Fluorescent detection — blue bands can be visualized with a 635 nm laser or red LED. Pink bands can be visualized with a 532 nm laser or green LED.

 

MW size range  10–250 kD 
Stain type Stained
Volume 250 µl
Number of applications  50 
Loading buffer  30% (w/v) glycerol, 2% SDS,
62.5 mM Tris, pH 6.8, 50 mM DTT,
5 mM EDTA, 0.02% NaN3,
0.01% bromophenol blue
Shelf life 12 months at –20°C
Precision Plus Protein™ WesternC™ Pack
Precision Plus Protein™ WesternC™ Pack

161-0385
1 pkg each Precision Plus Protein WesternC standards (#161-0376) and StrepTactin-HRP conjugate (#161-0381), 50 applications

Prezzo di listino:   263,00 €
Il tuo prezzo:   Accedi
Quantità:   Aggiungi a carrello
elenco preferiti   offerta
Precision Plus Protein™ WesternC™ Pack Value Pack

161-0398
1.25 ml (5 x 250 µl) Precision Plus Protein WesternC standards (#161-0376) and 625 µl (5 x 125 µl) StrepTactin-HRP conjugate (#161-0381), 250 applications 

Prezzo di listino:   1.052,00 €
Il tuo prezzo:   Accedi
Quantità:   Aggiungi a carrello
elenco preferiti   offerta
Precision Plus Protein™ WesternC™ Standards
Precision Plus Protein™ WesternC™ Standards

161-0376
250 μl, 10 blue-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications

Prezzo di listino:   200,00 €
Il tuo prezzo:   Accedi
Quantità:   Aggiungi a carrello
elenco preferiti   offerta
Precision Plus Protein™ WesternC™ Standards Value Pack

161-0399
1.25 ml (5 x 250 µl), 10 blue-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 250 applications

Prezzo di listino:   800,00 €
Il tuo prezzo:   Accedi
Quantità:   Aggiungi a carrello
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Precision Protein™ StrepTactin-HRP Conjugate
Precision Protein™ StrepTactin-HRP Conjugate

161-0380
300 μl, StrepTactin and horseradish peroxidase (HRP) conjugate used for detection of Precision Plus Protein unstained standards, 150 applications

Prezzo di listino:   144,00 €
Il tuo prezzo:   Accedi
Quantità:   Aggiungi a carrello
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Precision Protein™ StrepTactin-HRP Conjugate
Precision Protein™ StrepTactin-HRP Conjugate

161-0381
125 μl, StrepTactin and horseradish peroxidase (HRP) conjugate used for detection of Precision Plus Protein unstained standards, 50 applications

Prezzo di listino:   72,00 €
Il tuo prezzo:   Accedi
Quantità:   Aggiungi a carrello
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Precision Protein™ StrepTactin-AP Conjugate
Precision Protein™ StrepTactin-AP Conjugate

161-0382
300 μl, StrepTactin and alkaline phosphatase (AP) conjugate used for detection of Precision Plus Protein unstained standards, 150 applications

Prezzo di listino:   112,00 €
Il tuo prezzo:   Accedi
Quantità:   Aggiungi a carrello
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Number Descrizione Opzioni
6533 Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A Click to download
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
6040 Electrophoresis Guide, Interactive PDF, Rev B Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
5763 Molecular Weight Estimation Using Precision Plus Protein WesternC Standards on Criterion Tris-HCl and Criterion XT Bis-Tris Gels, Rev B Click to download
5723 Increase Western Blot Throughput with Multiplex Fluorescent Detection, Rev B Click to download
5685 Effect of PMA on Phosphorylation of Cx43: A Quantitative Evaluation Using Blotting With Multiplex Fluorescent Detection, Rev B Click to download
5576 Molecular Weight Estimation and Quantitation of Protein Samples Using Precision Plus Protein WesternC Standards, the Immun-Star WesternC Chemiluminescent Detection Kit, and the Molecular Imager ChemiDoc XRS Imaging System, Rev C Click to download [ Add to Cart (Free) ]
5575 Comparison of Chemiluminescent Protein Standards Product Insert, Rev C Click to download [ Add to Cart (Free) ]
5572 Immun-Star WesternC Chemiluminescent Kit Product Information Sheet, Rev D Click to download [ Add to Cart (Free) ]
5561 Precision Plus Protein WesternC Standards, Rev E Click to download [ Add to Cart (Free) ]
4110000 Instruction Manual, Precision StrepTactin HRP-Conjugate and AP-Conjugate, Rev D Click to download
10008761 Instruction Manual, Precision Plus Protein WesternC Standards, Rev F Click to download
2414 The Little Book of Standards, Rev D Click to download [ Add to Cart (Free) ]
2998 Protein Standards Application Guide, Rev G Click to download [ Add to Cart (Free) ]