Fast PCR Protocol Templates
The following PCR protocol templates can be quickly programmed into your cycler to help you get started with fast PCR.
Two-step fast PCR protocol using iTaq polymerase
(iTAQ-FST Protocol)
| 98°C for 30 sec | ||
| 92°C for 1 sec | } | x 35 cycles |
| 70°C for 10 sec* | ||
| 72°C for 15 sec |
*Optimal annealing extension times will be 10–30 sec for targets up to 500 bp.
Two-step PCR protocol using iProof polymerase for
long (1 kb) targets
(iPRF1kb Protocol)
| 95°C for 2 min | ||
| 95°C for 5 sec | } | x 20 cycles |
| 72°C for 30 sec | ||
| 72°C for 10 min |
Two-step PCR protocol using iProof polymerase for
longer (8 kb) targets
(iPRF8kb Protocol)
| 95°C for 2 min | ||
| 95°C for 5 sec | } | x 20 cycles |
| 72°C for 4 min | ||
| 72°C for 10 min |
Two-step PCR protocol using iProof polymerase for
extra long (15 kb) targets
(iPRF15kb Protocol)
| 95°C for 2 min | ||
| 95°C for 5 sec | } | x 20 cycles |
| 72°C for 7 min, 30 sec | ||
| 72°C for 10 min |