Chromatography Resin Information Library

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Bio-Rad manufactures a wide range of chromatography resins for process separations, providing process developers with great flexibility and high productivity. Our leading chromatography resins for biomolecule purifications include best-in-class ion exchange resins and our innovative mixed-mode resins.

  • Nuvia™ Ion Exchange Resins provide ultra–high binding capacity and exceptional resolution across a range of pH and flow rates — ideal for biosimilar and biotherapeutic downstream processes.
  • UNOsphere™ Ion Exchange Resins are highly macroporous beads allowing fast linear velocities at low backpressure — designed for high-efficiency capture of biomolecules from crude feedstreams.
  • CHT™ and Nuvia™ cPrime™ Mixed-Mode Resins offer unique separation properties and unparalleled selectivity and resolution for a variety of molecules. Combining ion exchange and metal affinity or hydrophobic interactions that enable the separation of biomolecules that appear homogeneous using other methods. These resins can be used at any stage in a purification process.

Ion Exchange Resins: Macro-Prep®, UNOsphere™, and Nuvia™ Matrices

Bio-Rad's ion exchange resins are available in various particle sizes, ionic forms, and purity ranges to offer a variety of purification solutions for process applications. These resins are ideal for biotherapeutic purifications where high productivity, economical use, and process flexibility are critical parameters for success.

Number Description Download
6713 Process Resin Selection Guide Click to download
6129 Nuvia™ Q Anion Exchange Resin Click to download
5987 Nuvia™ S Cation Exchange Resin Click to download
6448 Nuvia™ HR-S High-Resolution Cation Exchange Resin Click to download
2724 UNOsphere™ Q Anion Exchange Resin Click to download
2729 UNOsphere™ Q Anion Exchange Resin Technical Data Click to download
2669 UNOsphere™ S Cation Exchange Resin Click to download
2678 UNOsphere™ S Cation Exchange Resin Technical Data Click to download
5515 Guidelines for Using UNOsphere™ S Cation Exchange Resin Click to download
5644 Macro-Prep® High Q Anion Exchange Resin Click to download
5643 Macro-Prep® High S Cation Exchange Resin Click to download
Number Description Download
RP0048 How to Choose an Industrial Cation Exchanger for IgG Purification Click to download
RP0049 The Effect of Conductivity on Dynamic Binding Capacity on CEX Resins Tech Poster Click to download
6439 Improving Aggregate Removal from a Monoclonal Antibody Feedstream Using High-Resolution Cation Exchange Chromatography Click to download
6128 Workflow for Protein Purification from Whey Using Nuvia™ Ion Exchange Media Click to download
5984 Nuvia™ S Media: A High-Capacity Cation Exchanger for Process Purification of Monoclonal Antibodies Click to download
5735 Purification of a Monoclonal Antibody Using a Combination of UNOsphere™ Rapid S and UNOsphere Q Media Ion Exchange Chromatography after Protein A Capture Click to download
1942 Macro-Prep® DEAE Support for Chromatography of Biomolecules Click to download
2774 Purification of Transgenic Antibody from Corn Seed Using UNOsphere™ S and CHT™ Ceramic Hydroxyapatite Resins Click to download
2524 Purification of Horse IgG Using Macro-Prep® DEAE and CHT™ Ceramic Hydroxyapatite Type I Resins Click to download
1985 Clearance of Murine Leukemia Virus from a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography Click to download
1913 Developing and Scaling Up a Purification Procedure With Two New Ion Exchange Resins (Poster) Click to download
2204 Effective Removal of Negatively Charged Interfering Molecules From Proteins Click to download
1917 Purification of Human Monoclonal IgM Antibody from Bioreactor Supernatant Using a Combination of Cation and Anion Exchange Chromatography Click to download
6711 Purification of Amyloidogenic Transthyretin Protein Using Nuvia™ Q Anion Exchange Resin Click to download
6720 Development of a cGMP-Ready Purification Process for Adenovirus Purification (Poster) Click to download
Number Description Download
10033316 Instruction Manual, Nuvia™ HR-S Strong Cation Exchange Resins Click to download
10018215 Instruction Manual, Nuvia™ S and Q High-Capacity Ion Exchange Resins Click to download
4110109 Instruction Manual, UNOsphere™ Q and S Ion Exchange Resins Click to download
LIT271 Instruction Manual, Macro-Prep® Ion Exchange Resins Click to download

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Mixed-Mode Resins: Ceramic Apatite and Nuvia™ Matrices

Bio-Rad's mixed-mode chromatography resins offer unique separation properties and unparalleled selectivity and resolution for a variety of molecules. Their unique selectivities allow for purification of complex feeds and the separation of closely related species simply by manipulating a few conditions in one chromatography step. These resins can be used at any stage of purification, from capture to polishing.

Number Description Download
6713 Process Resin Selection Guide Click to download
6086 Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up Click to download
6242 Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin Click to download
5667 CHT™ Ceramic Hydroxyapatite Click to download
2156 CHT™ Ceramic Hydroxyapatite – A New Dimension in the Chromatography of Biological Molecules Click to download
6432 MPC™ Ceramic Hydroxyfluoroapatite Resin Click to download
3111 CFT™ Ceramic Fluoroapatite Resin Click to download
Number Description Download
6086 CHT™ Ceramic Hydroxyapatite Resource Guide – available online at www.bio-rad.com/chtguide Click to download
RP0033 A Ceramic Hydroxyapatite-Based Purification Platform Click to download
6549 Mammalian Virus Purification Using Ceramic Hydroxyapatite Click to download
6242 Purification of Recombinant Proteins on Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin: A Simple Approach to Method Development Click to download
6278 High-Throughput Calcium Analysis of Hydroxyapatite Column Effluent Click to download
6241 A Purification Strategy for Clinical-Grade Monoclonal Antibody Using Hydrophobic Cation Exchange Chromatography Click to download
6067 EDTA Complexometric Titration of Hydroxyapattite Column Effluent Click to download
5739 Packing and Slurry-in-Place Procedures for CHT™ Resin using Bio-Rad® InPlace™ Chromatography Columns Click to download
6418 Purification of Recombinant Proteins on Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin: A Simple Approach to Method Development Click to download
2204 Effective Removal of Negatively Charged Interfering Molecules from Proteins Click to download
2780 Purification of Murine IgG1 Using UNOsphere™ S and CHT™ Ceramic Hydroxyapatite Chromatography Click to download
2524 Purification of Horse IgG Using Macro-Prep® DEAE and CHT™ Ceramic Hydroxyapatite Type I Resins Click to download
2774 Purification of Transgenic Antibody from Corn Seed Using UNOsphere™ S and CHT™ Ceramic Hydroxyapatite Supports Click to download
2940 Removal of Aggregate From an IgG4 Product Using CHT™ Ceramic Hydroxyapatite Resin Click to download
2849 Protein A Removal from IgG on CHT™ Ceramic Hydroxyapatite Resin Click to download
2535 Purification of Recombinant Human Alpha 1-Antitrypsin From Transgenic Milk Using CHT™ Ceramic Hydroxyapatite Resin Click to download
2575 Purification of Calcium-Binding Protein using CHT™ Resin Click to download
2946 Plasmid DNA Adsorption Behavior on CHT™ Type II Resin Click to download
2644 CHT™ Ceramic Hydroxyapatite Resin: Use In Expanded-Bed Adsorption Mode Click to download
5853 Chimeric IgG Monoclonal Antibody Purification: A Comparative Study Using CHT™ and CFT™ Chromatography Resins Click to download
1986 Effect of pH on Gradient Elution of Proteins on Two Types of CHT™ Ceramic Hydroxyapatite Resin Click to download
5913 Separation of Fab and Fc Fragments from mAb Papain Digest on Ceramic Hydroxyapatite and Ceramic Fluoroapatite Resins Click to download
6720 Development of a cGMP-Ready Purification Process for Adenovirus Purification (Poster) Click to download
Number Description Download
6086 CHT™ Ceramic Hydroxyapatite Resource Guide - available online at www.bio-rad.com/chtguide Click to download

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Hydrophobic Resins: Macro-Prep® and Bio-Beads™ Matrices

Macro-Prep® Hydrophobic Interaction Chromatography (HIC) Resins are used for protein, polypeptide, enzyme, and nucleic acid purification. These resins are designed specifically for intermediate purification steps to remove host-cell contaminants from partially purified targets. Due to the rigidity and unique surface chemistry of these resins, they are well-suited for HIC operations requiring high throughput and high recovery of target.

Number Description Download
6713 Process Resin Selection Guide Click to download
1841 Macro-Prep® HIC Resin Click to download
1461 Bio-Beads™ SM-2 Hydrophobic Adsorbents Application Bibliography Click to download
1942 Macro-Prep® DEAE Support for Chromatography of BioMolecules Click to download
Number Description Download
LIT486 Instruction Manual, Macro-Prep® HIC Resins Click to download
LIT195 Instruction Manual, Bio-Beads™ SM Hydrophobic and Polar Interaction Adsorbents Click to download

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Affinity Resins: UNOsphere™ Matrix

Ready-to-use activated affinity media and IMAC and protein A resins are available for affinity purification of biomolecules. Protein A resins can be used for different monoclonal antibody (mAb) purification needs. UNOsphere SUPrA™ Resin is designed for process-scale purification of mAbs.

Number Description Download
6713 Process Resin Selection Guide Click to download
5729 UNOsphere SUPrA™ Resin Click to download
6484 UNOsphere™ Affinity Columns PIS Click to download
Number Description Download
5728 Monocolonal Antibody Purification Using UNOsphere SUPrA™ Resin Click to download
6053 Application of UNOsphere SUPrA™ Resin, a New Protein A Affinity Chromatographic Support, for Both Laboratory-Scale and Industrial Antibody Purification Click to download
Number Description Download
10014430 Instruction Manual, UNOsphere SUPrA™ Affinity Chromatography Media Click to download
10003245 Instruction Manual, Profinity™ Epoxide Resin Click to download
10044307 Instruction Manual, Bio-Rad® Nuvia™ IMAC Resin Click to download

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