Prepacked Bio-Scale™ Mini Size Exclusion Cartridges: Process Separations: Bio-Rad

Prepacked Bio-Scale™ Mini Size Exclusion Cartridges

print Email

Bio-Scale™ Mini Bio-Gel^® P-6 Desalting Cartridges

Overview

Cartridges are prepacked with Bio-Gel P polyacrylamide gels for gel filtration to separate molecules by size. Ready to use cartridges are designed for use with BioLogic systems or any chromatography system.

Bio-Scale Mini Cartridge Selection Guide

Bio-Scale™ Mini Cartridge Selection Guide

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	5 x 1 ml	1 x 5 ml	5 x 5 ml
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	–N⁺(CH₃)₃	≥37 mg BSA	732-4120	732-4122	732-4124
UNOsphere™ Q	UNOsphereQ	Strong anion exchange	–N⁺(CH₃)₃	180 mg BSA	732-4100	732-4102	732-4104
UNOsphere S	UNOsphere S	Strong cation exchange	–SO₃^-	60 mg human lgG	732-4110	732-4112	732-4114
DEAE	Macro-Prep DEAE	Weak anion exchange	–HN⁺(C₂H₅)₂	>35 mg protein	732-4140	732-4142	732-4144
Protein Purification
High S	Macro-Prep High S	Strong cation exchange	–SO₃^-	≥49 mg human lgG	732-4130	732-4132	732-4134
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum	—	732-4632	732-4634
Antibody Purification
Protein A	UNOsphere SUPrA™	Affinity	Recombinant protein A	30 mg/ml human IgG	732-4200, 1 x 1 ml	732-4201, 5 x 1 ml	732-4202, 1 x 5 ml
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal lgG_1; 16–23 mg human lgG	732-4600	732-4602	—
CHT Type I	CHT™ ceramic hydroxyapatite Type I, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg human lgG	—	732-4322	732-4324
CHT Type II	CHT ceramic hydroxyapatite Type II, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	15–25 mg human lgG	—	732-4332	732-4334
Affinity-Tagged Protein Purification
IMAC	Profinity™ IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant histidine (His)-tagged protein	732-4610	732-4612	732-4614
GST	Profinity GST	Affinity	Glutathione	≥10 mg/ml recombinant GST-tagged protein	732-4620	732-4622	732-4624
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel Blue	Affinity	Cibacron Blue F3GA	0.2 ml serum 11 mg albumin	—	732-4642	732-4644

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
					1 x 5 ml	5 x 5 ml	5 x 10 ml	1 x 50 ml	5 x 50 ml
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel filtration	Polyacrylamide	10–22% CV**	732- 4502	732- 4504	732- 5304	732- 5312	732- 5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

To read more click here

Cartridge Selection by application

Prepacked Cartridge Selection by Application

Cartridge by Application	Support	Type of Separation	Chemical Form	Binding Capacity
Protein and Plasmid Purification
UNOSphere™ Rapid S media	UNOSphere Rapid S	Strong cation exchange	SO₃-	Human IgG: 60 mg/ml*
CFT media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
Apatite purification kit	Ceramic hydroxyapatite Ceramic fluoropatite	Hydroxyapatite Fluoropatite	(Ca₅(PO₄)₃OH)₂ Ca₁₀(PO₄)₆F₂	Human IgG; 25-60 mg/ml** Lysozyme 15–21 mg/g
Protein Purification
UNOSphere Rapid S media	UNOSphere Rapid S	Strong cation exchange	SO₃-	Human IgG: 60 mg/ml*
CFT media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
Apatite purification kit	Ceramic hydroxyapatite Ceramic fluoropatite	Hydroxyapatite Fluoropatite	(Ca₅(PO₄)₃OH)₂ Ca₁₀(PO₄)₆F₂	Human IgG; 25–60 mg/ml** Lysozyme 15–21 mg/g
Antibody Purification
CFT Media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
UNOsphere SUPrA™ media	UNOsphere SUPrA	Affinity	Protein A
Monoclonal Antibody Purification
mAb purification kit	UNOsphere SUPrA CHT™ Type 1 UNOsphere Q	Affinity Hydroxyapatite Strong anion exchange	Protein A (Ca₅(PO₄)₃OH)₂ -N(CH₃)₃*	Human IgG: 25–60 mg/ml** BSA* 180 mg/ml

* Flow rate = 150 cm/hr.
** Flow rate = 300 cm/hr.

Bio-Scale Mini Cartridge Specifications

Properties of Bio-Gel P Polyacrylamide Gel

Properties of Bio-Gel P Polyacrylamide Gels


	Hydrated Bead Size (µm)	Hydrated Bed Volume Mass Dry Gel	Typical Flow Rate (cm/hr)*	Molecular Weight Fractionation Range

Bio-Gel P–2, fine	45–90	3 ml/g	5–10	100–1,800
Bio-Gel P–2, extra fine	<45		<10
Bio-Gel P–4, medium	90–180	4 ml/g	15–20	800–4,000
Bio-Gel P–4, fine	45–90		10–15
Bio-Gel P–4, extra fine	<45		<10
Bio-Gel P–6, medium	90–180	6.5 ml/g	15–20	1,000–6,000
Bio-Gel P–6, fine	45–90		10–15
Bio-Gel P–6, extra fine	<45		<10
Bio-Gel P–6DG gel	90–180		15–20
Bio-Gel P–10 gel, medium	90–180	7.5 ml/g	15–20	1,500–20,000
Bio-Gel P–10 gel, fine	45–90		10–15
Bio-Gel P–30 gel, medium	90–180	9 ml/g	7–13	2,400–40,000
Bio-Gel P–30 gel, fine	45–90		6–11
Bio-Gel P–60 gel, medium	90–180	11 ml/g	4–6	3,000–60,000
Bio-Gel P–60 gel, fine	45–90		3–5
Bio-Gel P–100 gel, medium	90–180	12 ml/g	4–6	5,000–100,000
Bio-Gel P–100 gel, fine	45–90		3–5

* Flow rates determined in a 1.5 70 column using a hydrostatic pressure head-to-bed ratio of 1:1.

Category Products

Bio-Scale™ Mini Bio-Gel^® P-6 Desalting Cartridges
These columns are 5 ml, 10 ml, and 50 ml Bio-Scale Mini cartridges filled with Bio-Gel P-6 support. They are used for desalting or buffer exchange using gel filtration (size exclusion) chromatography.

Specifications

Sizes	1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate	Varies for each support type*
Maximum operating pressure	1, 5, and 10 ml: 45 psi 50 ml: 40 psi
Fittings	Female luer inlet and male luer outlet
Column material	Polypropylene
Frit material	Polyethylene (HDPE)
Dimensions	1 ml — 40 mm length x 5.6 mm inner diameter 5 ml — 40 mm length x 12.6 mm inner diameter 10 ml — 80 mm length x 12.6 mm inner diameter 50 ml — 80 mm length x 28.2 mm inner diameter

Please Log In

Not Registered?

Process Separations