Prepacked Bio-Scale Mini Affinity Cartridges: Process Separations: Bio-Rad

Prepacked Bio-Scale Mini Affinity Cartridges

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Overview

The cartridges are prepacked with various single or multi-affinity chromatography media. Profinity media are designed for metal-affinity and affinity-tagged protein purifications.

Bio-Scale Mini Cartridge Selection Guide

Bio-Scale™ Mini Cartridge Selection Guide

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	5 x 1 ml	1 x 5 ml	5 x 5 ml
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	–N⁺(CH₃)₃	≥37 mg BSA	732-4120	732-4122	732-4124
UNOsphere™ Q	UNOsphereQ	Strong anion exchange	–N⁺(CH₃)₃	180 mg BSA	732-4100	732-4102	732-4104
UNOsphere S	UNOsphere S	Strong cation exchange	–SO₃^-	60 mg human lgG	732-4110	732-4112	732-4114
DEAE	Macro-Prep DEAE	Weak anion exchange	–HN⁺(C₂H₅)₂	>35 mg protein	732-4140	732-4142	732-4144
Protein Purification
High S	Macro-Prep High S	Strong cation exchange	–SO₃^-	≥49 mg human lgG	732-4130	732-4132	732-4134
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum	—	732-4632	732-4634
Antibody Purification
Protein A	UNOsphere SUPrA™	Affinity	Recombinant protein A	30 mg/ml human IgG	732-4200, 1 x 1 ml	732-4201, 5 x 1 ml	732-4202, 1 x 5 ml
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal lgG_1; 16–23 mg human lgG	732-4600	732-4602	—
CHT Type I	CHT™ ceramic hydroxyapatite Type I, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg human lgG	—	732-4322	732-4324
CHT Type II	CHT ceramic hydroxyapatite Type II, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	15–25 mg human lgG	—	732-4332	732-4334
Affinity-Tagged Protein Purification
IMAC	Profinity™ IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant histidine (His)-tagged protein	732-4610	732-4612	732-4614
GST	Profinity GST	Affinity	Glutathione	≥10 mg/ml recombinant GST-tagged protein	732-4620	732-4622	732-4624
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel Blue	Affinity	Cibacron Blue F3GA	0.2 ml serum 11 mg albumin	—	732-4642	732-4644

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
					1 x 5 ml	5 x 5 ml	5 x 10 ml	1 x 50 ml	5 x 50 ml
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel filtration	Polyacrylamide	10–22% CV**	732- 4502	732- 4504	732- 5304	732- 5312	732- 5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

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Cartridge Selection by Application

Prepacked Cartridge Selection by Application

Cartridge by Application	Support	Type of Separation	Chemical Form	Binding Capacity
Protein and Plasmid Purification
UNOSphere™ Rapid S media	UNOSphere Rapid S	Strong cation exchange	SO₃-	Human IgG: 60 mg/ml*
CFT media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
Apatite purification kit	Ceramic hydroxyapatite Ceramic fluoropatite	Hydroxyapatite Fluoropatite	(Ca₅(PO₄)₃OH)₂ Ca₁₀(PO₄)₆F₂	Human IgG; 25-60 mg/ml** Lysozyme 15–21 mg/g
Protein Purification
UNOSphere Rapid S media	UNOSphere Rapid S	Strong cation exchange	SO₃-	Human IgG: 60 mg/ml*
CFT media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
Apatite purification kit	Ceramic hydroxyapatite Ceramic fluoropatite	Hydroxyapatite Fluoropatite	(Ca₅(PO₄)₃OH)₂ Ca₁₀(PO₄)₆F₂	Human IgG; 25–60 mg/ml** Lysozyme 15–21 mg/g
Antibody Purification
CFT Media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
UNOsphere SUPrA™ media	UNOsphere SUPrA	Affinity	Protein A
Monoclonal Antibody Purification
mAb purification kit	UNOsphere SUPrA CHT™ Type 1 UNOsphere Q	Affinity Hydroxyapatite Strong anion exchange	Protein A (Ca₅(PO₄)₃OH)₂ -N(CH₃)₃*	Human IgG: 25–60 mg/ml** BSA* 180 mg/ml

* Flow rate = 150 cm/hr.
** Flow rate = 300 cm/hr.

mAb Workflow

Cartridge Comparison

Bio-Scale Mini Cartridge Specifications

Category Products

Bio-Scale™ Mini Profinity™ IMAC Cartridges
Bio-Scale Mini Profinity IMAC Cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges used for the purification of recombinant histidine (His)-tagged proteins.

Bio-Scale™ Mini Profinity™ GST Cartridges
Bio-Scale™ Mini Profinity™ GST cartridges are 1 ml and 5 ml cartridges filled with Profinity GST support. They are used for the purification of glutathione-S-transferase (GST)-tagged proteins.

Bio-Scale™ Mini Profinity eXact™ Cartridges
Bio-Scale Mini Profinity eXact cartridges are 1 ml and 5 ml cartridges of Profinity eXact resin for on-column purification and cleavage of proteins using the Profinity eXact fusion-tag system.

Bio-Scale™ Mini Affi-Gel^® Blue Cartridge
Bio-Scale Mini Affi-Gel Blue cartridges are 5 ml cartridges filled with 50–100 mesh Affi-Gel Blue support for use in affinity chromatography. It is ideally suited for albumin removal.

Bio-Scale™ Mini DEAE Affi-Gel^® Blue Cartridges
Bio-Scale Mini DEAE Affi-Gel Blue cartridges are 5 ml of DEAE Affi-Gel Blue support, a bifunctional affinity gel containing Cibacron Blue F3GA dye covalently attached to DEAE Bio-Gel A.

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Sizes	1 ml and 5 ml bed volumes
Dimensions	1 ml: 40 mm length x 5.6 mm inner diameter
	5 ml: 40 mm length x 12.6 mm inner dimensions
Maximum pressure tolerance	45 psi
Recommended flow rates	1 ml: 1–2 ml/min (240–480 cm/hr)
	5 ml: 5–10 ml/min (140–480 cm/hr)
Maximum flow rate	1 ml: 6 ml/min (1440 cm/hr)
	5 ml: 20 ml/min (963 cm/hr)
Fittings	Female luer fitting inlet and male luer fitting outlet
Column material	Polypropylene
Frit material	Polyethylene (HDPE)
Shipping condition	20% ethanol
Storage Recommendation	20% ethanol
Autoclavability	Not autoclavable

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Process Separations