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Protein purification sampler packs offer a quick way to experiment with separation techniques when the protein to be purified has not yet been characterized.
Cibacron Blue
F3GA and DEAE
* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.** Column volume.
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* Flow rate = 150 cm/hr. ** Flow rate = 300 cm/hr.
Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.
Comparison of Bio-Scale Mini Cartridges to other affinity columns on the Profinia Protein Purification system. Yield (A) and purity (B) of various proteins purified using either Bio-Scale Mini Profinity IMAC cartridges () or commercially available prepacked columns HisTrap FF () or HisTrap HP () on the Profinia system. Rec1, 75 kD; Fih1, 37 kD; DHFR, 25 kD; SME1, 11 kD.
* Recommended flow rates are given for each support type in the instruction manual. ** Available for desalting cartridges only.
Analysis of plasmid DNA purified on UNOsphere Q support. Fractions were separated on a 0.8% agarose gel. Lane 1, 1 kb marker (catalog #170-8204); lane 2, crude lysate; lane 3, flowthrough (fractions 2–6); lane 4, fractions 9–18; lane 5, fractions 19–30; lane 6, fractions 31–35; lane 7, fraction 36.
Chromatogram of murine IgG1 purification on the UNOsphere S column during small-scale process development. Diluted cell culture (20 ml) was loaded onto an UNOsphere S 0.7 x 5 cm column at a flow rate of 600 cm/hr in 20 mM phosphate-citrate buffer, pH 4.0. The sample was eluted in 10 column volumes (CV) of a 0–0.5 M NaCl gradient, followed by 5 CV of 1 M NaCl in the same buffer. The column was then cleaned in 1 M NaOH. The double-headed arrow indicates the fractions (1 ml each) containing IgG1. Blue trace, A280; red trace, conductivity profile.
Hydroxyapatite contains two types of binding sites, positively charged calcium and negatively charged phosphate groups. These sites aredistributed regularly throughout the crystal structure of the matrix. Solute species dominantly interact through cation exchange via the Schematic representation of CHT binding mechanisms.
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