The Profinia Protein Purification System Simplifies Antibody Purification with Protein A
Protein A affinity purification (–) and desalting (–) profiles using 0.1 M citrate elution buffer.
Comparison of protein A elution profiles using 0.1 M glycine, pH 3.0 (–) and 0.1 M citrate, pH 3.0 (–) elution buffers.
Protein A purification profiles using three concentrations of glycine, pH 3.0 (0.1 M (–); 0.25 M (–); 0.5 M (–)) for elution.
Protein A affinity purification (–) profiles using 0.25 M glycine elution buffer.
Purification of IgG from human serum using the Profinia protein purification system. Left panels represent chromatograms from purifications of IgG using 1 ml of human serum. The Profinia system monitors the absorbance at 280 nm from the affinity and desalting cartridges. Panels on the right display images from SDS-PAGE analyses of protein fractions from each experiment. M, Precision Plus Protein standards; L, load; F, flowthrough; W, wash; E, elution. The gel in D includes IgG purified using 0.1 M citrate as the elution buffer for comparison.
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