Protein Assay Kits and Cuvettes: Life Science Research: Bio-Rad

Protein Assay Kits and Cuvettes

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Overview

Bio-Rad offers four colorimetric assays that allow measurement of proteins using modifications of the Bradford and Lowry methods. Also offered are disposable standard and semimicro plastic cuvettes.

Protein Assay Selection Guide

Protein Assay Comparison

Characteristics of Bio-Rad's Protein Assays
	Protein Assay
	Quick Start™ Bradford	Bio-Rad	DC™	RC DC™
Adapted from method of	Bradford (1976)	Bradford (1976)	Lowry et al. (1951)	Lowry et al. (1951)
Standard concentration assay
Sample volume	100 µl	100 µl	100 µl	100 µl
Linear range	0.125–1.5 mg/ml	0.2–1.5 mg/ml	0.125–1.5 mg/ml	0.125–1.5 mg/ml
Low-concentration assay
Sample volume	1 ml	800 µl	200 µl	200 µl
Linear range	1.25–25 µg/ml	1.25–25 µg/ml	5–250 µg/ml	5–250 µg/ml
Microplate assay sample volume	5 µl	10 µl	5 µl	*
Minimum incubation time	5 min	5 min	15 min	15 min
Assay wavelength	595 nm	595 nm	650–750 nm	650–750 nm

All assays are easy to use, require little reagent preparation, and give accurate and reproducible results.

* To adapt the RC DC assay to a microplate format, follow the micro test tube (microfuge tube) assay protocol in the RC DC instruction manual up to the centrifugation step where the supernatant is discarded. The pellet can then be transferred to the microplate, and the microplate assay protocol in the DC protein assay manual can be followed.

In a typical protein assay, a chemical reagent is added to a protein sample, producing a visible result, such as a color change in the sample solution. This color change is quantitated with a spectrophotometer or microplate reader, and compared to a standard curve of known concentrations of protein versus their absorbance after reaction with the reagent. The amount of protein in the unknown sample is determined by interpolation, reading the concentration of protein on the standard curve that corresponds to its absorbance.

Category Products

Bio-Rad Protein Assay
The Bio-Rad protein assay is a simple colorimetric assay for measuring total protein concentration and is based on the Bradford dye-binding method.

DC™ Protein Assay
The DC (detergent compatible) protein assay is a colorimetric assay for protein concentration following detergent solubilization. The reaction is similar to the well-documented Lowry assay.

Quick Start™ Bradford Protein Assay
The Quick Start Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution.

RC DC™ Protein Assay
The RC DC (reducing agent and detergent compatible) protein assay is a colorimetric assay for protein determination in the presence of reducing agents and detergents.

Disposable Cuvettes for Protein Assays
Standard 3.5 ml or semimicrovolume 1.5 ml polystyrene cuvettes, ideal for use with protein assays, are offered.

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