| | Freeze 'N Squeeze DNA
Gel Extraction Spin Columns | Micro Bio-Spin and
Bio-Spin Columns | PCR Kleen
Spin Columns |
| Application | Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels | Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting | Removal of PCR products >200 bp from other reaction mixture components |
| Starting Material | ≤700 mg gel slice | 10–100 µl of sequencing reaction mixture | 25–100 µl of PCR or other enzyme reaction |
| Format | Spin-column extraction of frozen-thawed gel slices | Prepacked size exclusion spin column | Prepacked size exclusion spin column |
| Recovery | 4–23 kb: >80% recovery;
0.5–4 kb: >70% recovery;
50–500 bp: >50% recovery | >95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators | 50–80% |
| Time Required | <10 min | <10 min | <4 min |
