Imaging System Selection Guide
Nucleic Acid Detection
| Ethidium bromide |
5 |
4 |
- |
4 |
4 |
5 |
5 |
- |
| SYBR® Green I stain |
5 |
4 |
- |
4 |
4 |
5 |
5 |
- |
| SYBR® Safe stain |
5 |
4 |
- |
4 |
4 |
5 |
5 |
- |
| Coumarin stain |
5 |
4 |
5 |
- |
- |
- |
- |
- |
| Fast Blast™ DNA stain |
4 |
4 |
5 |
4 |
4 |
- |
- |
- |
Protein Staining, 1-D Gels
| Stain-free gels |
5 |
5 |
- |
5 |
5 |
- |
- |
- |
| Coomassie Blue stain |
4 |
4 |
5 |
4 |
4 |
2 |
2 |
- |
| Silver stain |
4 |
4 |
5 |
4 |
4 |
2 |
2 |
- |
| SYPRO Ruby protein blot stain |
4 |
4 |
- |
4 |
4 |
5 |
5 |
- |
| Flamingo™ fluorescent gel stain |
4 |
3 |
- |
3 |
3 |
5 |
5 |
- |
| Oriole™ fluorescent gel stain |
5 |
4 |
- |
5 |
5 |
- |
- |
- |
Protein Staining, 2-D Gels
| Coomassie Blue stain |
4 |
3 |
5 |
3 |
3 |
2 |
2 |
- |
| Silver stain |
4 |
3 |
5 |
3 |
3 |
2 |
2 |
- |
| SYPRO Ruby protein blot stain |
4 |
3 |
- |
3 |
3 |
5 |
5 |
- |
| Flamingo™ fluorescent gel stain |
4 |
2 |
- |
3 |
3 |
5 |
5 |
- |
| Oriole™ fluorescent gel stain |
5 |
3 |
3 |
3 |
3 |
3 |
3 |
3 |
| Pro-Q stain |
4 |
2 |
- |
2 |
3 |
5 |
5 |
- |
| Cy2, Cy3, Cy5 label |
4 |
- |
- |
- |
- |
5 |
5 |
- |
Blot Detection
| Stain-free blots |
4 |
5 |
- |
- |
- |
- |
- |
- |
| Coomassie Blue stain |
4 |
- |
5 |
4 |
4 |
2 |
2 |
- |
| Silver stain |
5 |
- |
5 |
4 |
4 |
2 |
2 |
- |
| SYPRO Ruby protein blot stain* |
5 |
- |
- |
- |
- |
5 |
5 |
- |
| Immun-Star™ chemiluminescence |
5 |
- |
- |
- |
4 |
- |
- |
- |
| Chemifluorescence* |
5 |
- |
- |
1 |
1 |
4 |
4 |
- |
| Qdot* |
5 |
2 |
- |
2 |
2 |
5 |
5 |
- |
| Multiplex fluorescence |
5 |
- |
- |
- |
- |
- |
- |
- |
Micro- and Macroarray** Detection
| Radiolabel |
- |
- |
- |
- |
- |
- |
5 |
5 |
| Fluorescence |
5 |
- |
- |
2 |
2 |
5 |
5 |
- |
| Immun-Star™ chemiluminescence |
5 |
- |
- |
- |
4 |
- |
- |
- |
Colony Counting
| Colorimetric detection |
5 |
- |
- |
4 |
4 |
3 |
3 |
- |
| Fluorescent detection |
5 |
- |
- |
4 |
4 |
5 |
5 |
- |
Isotopic
| Radiolabel |
- |
- |
- |
- |
- |
- |
5 |
5 |
| X-ray film |
4 |
- |
5 |
4 |
4 |
3 |
3 |
- |
- Not recommended; 1–5, recommendation level (5 = highest).
* Optimal with low fluorescence PVDF membrane.
** With spot features ≥400 µm.