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Suites of chromatography media are offered in convenient sampler packs. Available in bulk as well as in prepacked columns, these media assortments are intended as aids in developing new purification workflows.
High-throughput experimentation (HTE) was used in this case study in concert with design of experiments (DOE) methodology to study the mass recovery of a basic therapeutic protein (pI 9.2–9.5) using a hydrophobic cation exchange mixed-mode resin.
Cibacron Blue
F3GA and DEAE
* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.** Column volume.
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Comparison of Bio-Scale mini cartridges to other affinity columns on the Profinia™ protein purification system. Yield (A) and purity (B) of various proteins purified using either Bio-Scale™ Mini Profinity™ IMAC cartridges () or commercially available prepacked columns HisTrap FF () or HisTrap HP () on the Profinia system. Rec1, 75 kD; Fih1, 37 kD; DHFR, 25 kD; SME1, 11 kD.
Bio-Scale Mini cartridges have a patent-pending double-wall design that provides extra durability and allows easy, reliable runs with aqueous buffers most commonly used for protein purification. The polypropylene Luer fittings and internal sealing surfaces assure leak-free operation, at pressures up to 45 psi. The cartridges are convenient, disposable, and supplied ready for use. They are easy-to-use, prepacked chromatographic cartridges for fast, reproducible chromatographic separations. Cartridges are available for a variety of chromatographic techniques including desalting, ion exchange, and affinity chromatography.
Cartridges are prepacked with Bio-Rad's UNOsphere and Macro-Prep chromatography media for ion exchange chromatography. Ready to use cartridges are designed for use with BioLogic systems or any.
The cartridges are prepacked with Bio-Rad's protein A affinity chromatography media for antibody purification. Ready to use cartridges are designed for use with BioLogic™ systems or any chromatography system.
The cartridges, prepacked with Bio-Rad's CHT™ ceramic hydroxyapatite and CFT™ ceramic fluoroapatite media, are convenient for evaluating performance of the different apatite media for a new application.
The cartridges are prepacked with various single or multi-affinity chromatography media. Profinity™ media are designed for metal-affinity and affinity-tagged protein purifications.
Cartridges are prepacked with Bio-Gel® P polyacrylamide gels for gel filtration to separate molecules by size. Ready-to-use cartridges are designed for use with BioLogic™ systems or any chromatography system.
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