Size Exclusion Chromatography Media

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Overview

Size exclusion or gel filtration chromatography media include Bio-Gel P polyacrylamide gels for a wide range of high molecular weight molecules and Bio-Beads S-X media for the separation of lipophilic polymers.

Size Exclusion Media

Size Exclusion Chromatography Media Selection Guide

Properties

	Hydrated Bead Size (µm)	Hydrated Bed Volume Mass Dry Gel	Typical Flow Rates (cm/hr)*	Molecular Weight Fractionation Range

Bio-Gel P-2 gel, fine Bio-Gel P-2 gel, extra fine	45–90 <45	3 ml/g	5–10 <10	100–1,800
Bio-Gel P-4 gel, medium Bio-Gel P-4 gel, fine Bio-Gel P-4 gel, extra fine	90–180 45–90 <45	4 ml/g	15–20 10–15 <10	800–4,000
Bio-Gel P-6 gel, medium Bio-Gel P-6 gel, fine Bio-Gel P-6 gel, extra fine Bio-Gel P-6DG gel	90–180 45–90 <45 90–180	6.5 ml/g	15–20 10–15 <10 15–20	1,000–6,000
Bio-Gel P-10 gel, medium Bio-Gel P-10 gel, fine	90–180 45–90	7.5 ml/g	15–20 10–15	1,500–20,000
Bio-Gel P-30 gel, medium Bio-Gel P-30 gel, fine	90–180 45–90	9 ml/g	7–13 6–11	2,500–40,000
Bio-Gel P-60 gel, medium Bio-Gel P-60 gel, fine	90–180 45–90	11 ml/g	4–6 3–5	3,000–60,000
Bio-Gel P-100 gel, medium Bio-Gel P-100 gel, fine	90–180 45–90	12 ml/g	4–6 3–5	50,000–100,000

* Flow rates determined in a 1.5 x 70 cm column using a hydrostatic pressure head-to-bed ratio of 1:1.

The chart provides a selection of Bio-Gel P media for Size exclusion chromatography, also known as gel filtration chromatography, which separates molecules by size. The gel matrix consists of spherical beads with pores of a specific size distribution. Separation occurs when molecules of different sizes are excluded from the pores. As small molecules diffuse into the pores, their flow through the column is retarded, while large molecules bypass the pores and are eluted in the column�s void volume. Consequently, molecules are separated according to size and elute in order of decreasing molecular weight.

Category Products

Bio-Gel P Polyacrylamide Gels
Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylene-bis-acrylamide.

Bio-Gel A Agarose Gel
Bio-Gel A 1.5m gel, ideal for purification of antibodies and aggregates, consists of agarose beads in which the pore size is controlled by the percentage of agarose.

Bio-Beads S-X Media
Bio-Beads S-X media are neutral, porous styrene divinylbenzene beads for size exclusion chromatography of lipophilic polymers and other solutes that require organic eluents.

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Size Exclusion Chromatography Media

Overview

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Size Exclusion Chromatography Media

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