Nucleic Acid Sample Preparation and Purification

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Overview

Complete line of kits and reagents for fast and effective extraction and purification of RNA, DNA, and PCR products. Small- and large-scale solutions are available for various downstream applications.

Plasmid Prep Selection Guide

RNA Isolation Selection Guide

	Aurum Total RNA Kits			PureZOL RNA Isolation Reagent
	Mini	Fatty and Fibrous Tissue	96	PureZOL RNA Isolation Reagent
Format	Mini column Filtration (vacuum or spin)	Mini column Filtration (vacuum or spin)	96-well plate Filtration (vacuum or spin)	—
Maximum starting amounts
Cultured cells	2 x 10⁶	—	1 x 10⁶	1 x 10⁷
Bacterial cells	2.4 x 10⁹	—	8 x 10⁸	2.4 x 10⁹
Yeast cells	3 x 10⁷	—	2 x 10⁷	3 x 10⁷
Animal tissue — hard	20 mg	100 mg	—	100 mg
Animal tissue — soft to moderately hard	40 mg	100 mg	—	100 mg
Plant tissue	40 mg	100 mg	—	100 mg
Isolation method	Silica membrane	Lysis with PureZOL reagent, purification on silica membrane	Silica membrane	Organic extraction
Number of preps	10* or 50 mini preps	10* or 50 mini preps	2 x 96-well plate	50 or 100 preps (1 ml/prep)
Number of washes	3	3	3	—
DNase I included**	Yes	Yes	Yes	No
DNase I digest time	15 min (animal tissue, 25 min)	15 min	10 min	—
Total preparation time	<50min (1.5 hr with DNase I)	<50min (1.5 hr with DNase I)	<60 min	<60 min
Binding capacity	>100 µg	>100 µg	>40 µg	—
Elution volume	2 x 40 µl	2 x 40 µl	80 µl	30–100 µl
Yield***	28 µg/2 x 10⁶ NIH 3T3 cells	96 µg/100 mg brain	10 µg/1 x 10⁶ NIH 3T3 cells	1.15 µg/mg brain
	38 µg/2 x 10⁶ HeLa cells	58 µg/100 mg breast	17 µg/1 x 10⁶ HeLa cells	1.37 µg/mg fat
	20 µg/40 mg brain	85 µg/100 mg heart	11 µg/2 x 10⁷ S. cerevisiae cells	1.2 µg/mg heart
	52 µg/20 mg thymus	63 µg/100 mg skin	5 µg/8 x 10⁸ E. coli cells	0.66 µg/mg cartilage
	132 µg/40 mg spleen	54 µg/100 mg cartilage	5 µg/8 x 10⁸ B. cereus cells	1.2 µg/mg skin
	36 µg/40 mg lung			1.0 µg/mg potato
	32 µg/40 mg potato			1.37 µg/1 x 10⁵ 293H cells
	28 µg/3 x 10⁷ S. cerevisiae cells			0.1 µg/mg Arabidopsis
	54 µg/2.4 x 10⁹ E. coli cells			0.38 µg/mg A. niger
				0.63 µg/2.4 x 10⁷ E. coli cells
				0.31 µg/3 x 10⁵ S. cerevisiae cells
Purity	A₂₆₀/A₂₈₀ of 1.9–2.1	A₂₆₀/A₂₈₀ of 1.9–2.1	A₂₆₀/A₂₈₀ of 1.9–2.1	A₂₆₀/A₂₈₀ of 1.9–2.1

* Trial sizes of the Aurum total RNA mini kit and Aurum total RNA fatty and fibrous tissue kit are available.

** Removal not required.

*** Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.

PCR/DNA Purification Guide

Category Products

RNA Isolation
Bio-Rad provides two distinct technologies for isolating total RNA. Silica membrane-based kits, available in 96-well plate and spin-column formats, and PureZOL RNA reagent ideal for scaling up isolation ...More »

DNA Isolation
DNA preparation is made fast and easy with Bio-Rad's plasmid and PCR-ready genomic DNA extraction products. A choice of solutions is available for a variety of starting sample types.

DNA Cleanup
Bio-Rad offers various kits for purifying PCR products and other DNA molecules directly from reaction mixtures and for extracting and purifying DNA fragments from agarose gels.

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Kit	DNA Yield	Preparation Time	Bacterial Culture Density or Volume	Purification Format	Binding Matrix	Growth Media
Aurum plasmid mini	>20µg	10 min	Up to 12 OD • ml*	Vacuum** and spin	Silica membrane	Standard
Quantum Prep miniprep	>20µg	15 min	1–10 ml	Spin	Diatomaceous earth	Enriched or standard

	Freeze 'N Squeeze DNA Gel Extraction Spin Columns	Micro Bio-Spin and Bio-Spin Columns	PCR Kleen Spin Columns
Application	Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels	Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting	Removal of PCR products >200 bp from other reaction mixture components
Starting Material	≤700 mg gel slice	10–100 µl of sequencing reaction mixture	25–100 µl of PCR or other enzyme reaction
Format	Spin-column extraction of frozen-thawed gel slices	Prepacked size exclusion spin column	Prepacked size exclusion spin column
Recovery	4–23 kb: >80% recovery; 0.5–4 kb: >70% recovery; 50–500 bp: >50% recovery	>95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators	50–80%
Time Required	<10 min	<10 min	<4 min

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Nucleic Acid Sample Preparation and Purification

Overview

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Nucleic Acid Sample Preparation and Purification

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