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Traditional semi-dry transfer system transfers proteins ranging from 10–100 kD in 15–60 minutes. Trans-Blot Turbo system delivers efficient and reproducible transfer of proteins up to 400 kD in as little as 3 minutes.
Higher sensitivity and better transfer efficiency is seen with the Trans-Blot® Turbo™ system in comparison to other blotting techniques. This data set demonstrates the successful transfer of the 1.25 ng protein band only with the Trans-Blot Turbo system. Serial dilutions of transferrin were separated on a Criterion™ TGX™ 4–20% gel and transferred using four different blotting techniques. All images are adjusted to the same settings.
2x Protein Transfer
Quantitation performed on equivalent 2-D gels transferred with the Trans-Blot® Turbo™ and the iBlot systems demonstrated twice the number of proteins transferred and detected with the Trans-Blot Turbo system. Rat liver extract was focused on Bio-Rad's ReadyStrip™ 11 cm pH 5–8 IPG strips and separated on AnykD™ Criterion™ TGX™ gels. Duplicate gels were transferred with the Trans-Blot Turbo and iBlot systems, both using manufacturers’ recommended 7 min protocol. The nitrocellulose membranes were subsequently stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system.
Turbo Transfers with Trans-Blot® Turbo™ Transfer Packs
E. coli lysate (6 µg) was diluted two-fold. Samples were separated with Mini-PROTEAN TGX gels, transferred with the Trans-Blot Turbo system, stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system. Standards in lane 1 are Precision Plus Protein™ Unstained standards, with a top band of 250 kD MW.
Instructional video to guide you through the features, benefits, and operation of the Trans-Blot Turbo system.
Bio-Rad Trans-Blot SD Semi-Dry Blotting Apparatus
Trans-Blot SD Semi-Dry Assembly Guide
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