PCR Seals

• Microseal^® 'B' Adhesive Seals, Optical
• Microseal^® 'C' Optical Seals
• Microseal^® 'F' Foil
• Microseal^® 'A' Film
• Heat Sealing Films and Foils
• Optical Sealing Tape
• 96-Well Plate Sealing Mats
• Microseal^® 'P' and 'P+' Sealing Pads
• Auto-Sealing Lids for PCR Plates
• Chill-out™ Liquid Wax
• Frame-Seal™ Incubation Chambers for In Situ PCR and Hybridization

Overview

Bio-Rad offers several adhesive and heat sealing films for 96-well and 384-well plates that are adapted for different uses, and provides tools to facilitate their use.

Heat Sealing Films and Foils

Heat Sealing Films and Foils

	Optically Clear Heat Seal	Permanent Clear Heat Seal	Pierceable Foil Heat Seal	Peelable Foil Heat Seal
Catalog number	181-4030	181-4035	181-4040	181-4045
Applications	– Real-time PCR – PCR	– Water bath cycling – PCR	– QX100™ Droplet Digital™   PCR system – PCR	– Sample storage – PCR
Maximum temperature	110°C	110°C	110°C	110°C
Minimum temperature	–80°C	–80°C	–20°C	–200°C
Peelable seal	+		+	+
Pierceable seal			+
DNase and RNase free	+	+	+	+
Sealing compatibility	PP, COC, PE,* PS	PP	PP, PS*	PP, COC*
COC, cyclic olefin copolymer; PE, polyethylene; PP, polypropylene; PS, polystyrene. * Limited and variable compatibility based on plate composition.

Amplification Reagents and Plastics Brochure

Amplification Reagents and Plastics Brochure

The Amplification Reagents and Plastics Brochure includes information about

 

• Products for gene expression analysis
  

  RNA Isolation

  Reverse Transcription Reagents

  Real-Time qPCR Reagents

  PCR Plastic Consumables



• Product performance data


• Quick and easy product selection tables


• Ordering information

 

Download your free copy of the Amplification Reagents and Plastics Brochure.

PCR Consumables Selection Wizard

PCR Consumables Selection Wizard

Category Products

Microseal® 'B' Adhesive Seals, Optical Microseal 'B' seal is an adhesive-based sealing option for thermal cycling applications using thin-wall PCR plates. The aggressive adhesive layer ensures secure sample storage or transport.

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Microseal® 'C' Optical Seals Microseal 'C' optical seals are optically clear adhesive films designed for optical assays such as real-time PCR. They can be used for microplates with raised well rims.

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Microseal® 'F' Foil This aluminum foil acts as a barrier against evaporation from –70°C to 105°C. It is thin enough to pierce with a pipet tip for recovery of samples, and it is suitable for use with automated systems.

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Microseal® 'A' Film Microseal 'A' film is an effective means to seal the full range of Bio-Rad PCR plates and tubes. Designed to seal tightly during cycling, it releases smoothly to minimize the risk of cross-contamination.

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Heat Sealing Films and Foils Heat sealing films and foils efficiently seal microplates for standard, real-time, and digital PCR. These sealing solutions deliver reliable data by minimizing sample evaporation during thermal cycling.

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Optical Sealing Tape Optical sealing tape is an adhesive-based sealing option for optical assays demanding a low-fluorescent background. The tape is validated for real-time PCR on Bio-Rad's iCycler iQ® systems.

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96-Well Plate Sealing Mats PCR plate sealing mats are reusable, economical, and convenient for sealing PCR plates for oil-free thermal cycling reactions. An array of 96 bumps helps orient the rubber mat on the PCR plate.

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Microseal® 'P' and 'P+' Sealing Pads These reusable sealing pads are designed to adhere to a motorized heated lid. They are ideal for applications such as cycle sequencing, in which several successive runs may be sealed with the same pad.

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Auto-Sealing Lids for PCR Plates Auto-sealing lids are reusable, automation-friendly PCR plate sealers that prevent evaporation and contamination during reaction setup and seal tightly for PCR when the cycler lid is closed.

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Chill-out™ Liquid Wax Chill-out liquid wax provides an excellent vapor barrier that may be used instead of mineral oil in thermal cyclers without heated lids. After cycling, the tubes are chilled to harden the wax.

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Frame-Seal™ Incubation Chambers for In Situ PCR and Hybridization Frame-Seal incubation chambers are easy-to-use, strongly adhesive hybridization chambers with flexible plastic coverslips. They provide vapor-tight sealing for FISH, polonies, in situ PCR, and PRINS.

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