The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.
Stephen A SA Bustin, Vladimir V Benes, Jeremy A JA Garson, Jan J Hellemans, Jim J Huggett, Mikael M Kubista, Reinhold R Mueller, Tania T Nolan, Michael W MW Pfaffl, Gregory L GL Shipley, Jo J Vandesompele, Carl T CT Wittwer
Audio, Transactions of the IRE Professional Group on
Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments.