qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data.
Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J
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Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.