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2-DE is an essential step in biomarker discovery workflow, whether your goal is protein characterization, purification and profiling, or posttranslational modification studies.
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How can automation in electrophoresis enhance reliability in experiments assessing the purity and yield of proteins or evaluating the integrity of RNA?
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Details on how biomarkers such as hormones, cytokines, chemokines, growth factors, and phosphoproteins are used to investigate physiological or pharmacological mechanisms.
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Explore different aspects of genomics — from functional genomics and biomarker discovery to mutation analysis, gene expression, transfection, and epigenetics.
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Various aspects of how to develop, optimize, and validate biomolecular interaction assays using surface plasmon resonance (SPR) technology.
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Bio-Rad’s V3 Western Workflow™ facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.
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Tips for isolation, maintenance, differentiation, transfection and analysis of stem cells.
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Explore applications of chromatography: recombinant protein purification, monoclonal antibody discovery, viral contaminant removal, protein isolation, and more.
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Protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.
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Various aspects of how PCR works, how to choose the right PCR instrument, how to design, optimize, analyze, and troubleshoot PCR assays.
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Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.
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An array of solutions to optimize, carry out, and troubleshoot MIQE-compliant real-time PCR experiments.
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Comparison of parameters, sensitivity, and data analysis using Bio-Rad imaging systems and software to determine which imaging system is ideal for your application.
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Information on the digital PCR technology, and tips on planning droplet digital PCR experiments as well as on absolute quantification of targets using ddPCR can be found here.
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Useful information from sample preparation to imaging of 2-D gels, along with protocols, video tutorials, demonstrations, and troubleshooting tips.
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Information and tips on electroporation, viral transfection vector, lipofection, and biolistics transformation for planning and selecting the appropriate transfection method.
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Gel boxes, running buffers, available agarose types, as well as factors affecting resolution of DNA fragments, to help with agarose gel electrophoresis of DNA.
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Manual and automated cell counting methods and their application to gene expression studies are presented and discussed here.
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How can large DNA fragments be separated using PFGE? How to prepare plugs, optimize sample resolution, analyze images, and troubleshoot PFGE gels? Explore.
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Details on the experimental design, data collection, analysis, and validation of multiplex protein assays using the Bio-Plex® suspension array system.
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The microfluidic technology used in Bio-Rad’s Experion™ system helps to automate protein and nucleic acid separation, detection, and analysis.
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How does this label-free system perform analysis of biomolecular interactions? How do you choose the right sensor chip for your analysis? Find out.
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Description of the different types of chromatography methods available and how they can be applied to guide you in your protein purification experiments.
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