Life Science Research

Bio-Rad Releases "The PCR Song": Scientists for Better PCR

As mentioned by Nature.com, The New York Times online, and blogs across the world, Bio-Rad released "The PCR Song", a tribute to all the scientists performing PCR.

Lyrics for "The PCR Song"

There was a time when to amplify DNA,
You had to grow tons and tons of tiny cells.

Then along came a guy named Dr. Kary Mullis,
Said you can amplify in vitro just as well.

Just mix your template with a buffer and some primers,
Nucleotides and polymerases, too.

Denaturing, annealing, and extending.
Well it’s amazing what heating and cooling and heating will do.

PCR, when you need to detect mutations.
PCR, when you need to recombine.
PCR, when you need to find out who the daddy is.
PCR, when you need to solve a crime.

(repeat chorus)

Over 20 Years of Innovation

From the introduction of the PTC-100^® in 1988 to the launch of the C1000 Touch™ thermal cycler in 2011, Bio-Rad Laboratories has led the way in thermal cycler innovation for over 20 years. Bio-Rad's unique family of products for PCR research gives you the power to do the experiments you want to do, the way you want to do them.

	2011 — CFX96 Touch™ and CFX384 Touch™ Real-Time PCR Detection Systems The CFX96 Touch and CFX384 Touch systems introduce a new level of user friendliness with the inclusion of an integrated touch screen in the C1000 Touch thermal cycler chassis. The systems feature easy run setup using streamlined menu navigation and, in an industry first, monitoring of real-time amplification traces without a computer attached.
	2011 — CFX Connect™ Real-Time PCR Detection System The CFX Connect system delivers excellent thermal and optical performance for all SYBR® Green/EvaGreen and duplex experiments in a 96-well format. Utilizing the powerful, easy-to-use CFX Manager™ software, the CFX Connect system offers a complete package to analyze even the most challenging experiments.
	2011 — C1000 Touch™ Thermal Cycler The C1000 Touch thermal cycler builds on the advanced performance and features of the C1000™ thermal cycler by adding a large color touch screen and redesigned interface. This thermal cycler is compatible with six interchangeable reaction modules, including two for real-time PCR.
	2011 — T100™ Thermal Cycler The compact 96-well T100 thermal cycler offers a comprehensive package of features, including an easy-to-use touch screen, thermal gradient, and reliable performance.
	2008 — CFX96™ and CFX384™ Real-Time PCR Instrumentation The CFX96 and CFX384 real-time PCR detection systems bring flexibility and ease of use to researchers performing high-throughput real-time PCR in a 96- or 384-well format. The solid state components provide sensitive detection for precise quantification and reliable performance.
	2008 — 1000-Series of PCR Instrumentation The 1000-series includes the C1000 thermal cycler with innovative features such as the protocol autowriter, the S1000™ thermal cycler, and the high-performance six-channel CFX96 and five-channel CFX384 real-time PCR detection systems. This platform has a patented, reduced-mass sample block for faster time to target temperature and the only gradient-enabled dual block on the market.
	2005 — iQ™5 Real-Time PCR Detection System The iQ5 system offers five-target real-time PCR detection, powerful data analysis options, and advanced multiplex performance to researchers seeking a highly flexible system for real-time PCR analysis.
	2005 — MJ Mini™ Personal Thermal Cycler The compact yet powerful 48-well MJ Mini cycler boasts features found in larger instruments, including unparalleled thermal performance and thermal gradient technology.
	2005 — MiniOpticon™ Two-Color Real-Time PCR Detection System The MiniOpticon two-color real-time PCR detector is the most compact real-time PCR system available. It uses a fixed array of 48 LEDs and photodiodes for sensitive, reliable quantification.
	2004 — Chromo4™ Real-Time PCR Detection System The Chromo4 detector offers users innovative technology in a compact design, bringing four-color real-time detection capability and options for increased throughput to the DNA Engine® thermal cycler.
	2003 — MyiQ™ Real-Time PCR Detection System The single-color MyiQ real-time PCR detection system is an affordable option for new users of real-time PCR technology as well as those who must handle increasing workload demands involving gene expression. This real-time PCR system is a modular upgrade to the highly flexible iCycler® thermal cycler.
	2002 — MyCycler™ Personal Thermal Cycler The MyCycler thermal cycler set a new standard for personal cyclers with a high-resolution graphical display, intuitive graphic-based programming, and exceptional thermal performance.
	2002 — DNA Engine Opticon® 2 Real-Time PCR Detection System The DNA Engine Opticon 2 system has the same standard features as the original DNA Engine Opticon system, with the added capability of two-color detection using a fixed array of 96 aqua-colored LEDs and two PMTs for detection.
	2001 — DNA Engine Opticon® Real-Time PCR Detection System The novel DNA Engine Opticon detector enables quantitative and melt curve analysis as well as other precision temperature–controlled measurements on the DNA Engine thermal cycler. Its unique rugged design uses LED excitation and PMT detection to deliver high sensitivity with no moving parts.
	2000 — DNA Engine Dyad® Thermal Cycler The dual-bay DNA Engine Dyad cycler added ease-of-use features, such as a window-driven interface, point-and-click programming, real-time temperature graphs, and a high-density color display, to the range of compatible options for tens of thousands of DNA Engine owners.
	1999 — iCycler iQ® Real-Time PCR Detection System The iCycler iQ system brought the first modular upgrade to the real-time PCR market. It features four-target detection, the use of filtered excitation from a tungsten halogen lamp, and filtered emission detected by a high-pixel-density CCD camera.
	1999 — iCycler® Thermal Cycler The iCycler thermal cycler is a state-of-the-art thermal cycler developed to provide the highest performance standards and easiest user interface on the market. Interchangeable reaction blocks, unparalleled ease of use via the large VGA screen, and an upgrade path to real-time PCR make the iCycler instrument the most innovative and flexible thermal cycler available.
	1999 — Thermal Gradient Feature To help researchers optimize reactions, we offered a thermal gradient feature to combine unsurpassed accuracy with sophisticated dynamic ramping. Efficient empirical determination of annealing and denaturation temperatures has never been easier or more precise.
	1997 — Remote Alpha Dock™ System The Remote Alpha Dock system was the first to address the demand for hands-free unattended amplification by offering automation-friendly options for thermal cycling. The Remote Alpha Dock system and Power Bonnet™ automated lid (first offered in 1996) offer creative solutions to integrating thermal cyclers into robotic systems.
	1996 — Slide Chambers™ Dual Alpha™ Unit In 1996, we adapted slide-cycling technology for microscope slides to the DNA Engine line of cyclers by developing the Slide Chambers unit. This Alpha™ unit has the capacity to independently control two 16-chamber slide towers, making it ideal for researchers wishing to add slide cycling or microarray hybridization capability to an existing DNA Engine cycler.
	1995 — DNA Engine Tetrad® Thermal Cycler The DNA Engine family grew, adding an instrument that incorporated four DNA Engine cyclers into a single, minimal footprint. We also designed and introduced the configuration for 384-well blocks and vessels, which became the standard in high-throughput DNA sequencing and the thermal cycling backbone of the Human Genome Project.
	1994 — DNA Engine® Thermal Cycler Our first user-configurable cycler introduced swappable blocks accommodating a wide variety of vessel formats. Its revolutionary multisensor, multizone, thermal control delivers superior thermal uniformity and unprecedented speed. The DNA Engine architecture allows new block types to be added as research needs evolve.
	1991 — MiniCycler® Personal Thermal Cycler The first personal-sized cycler set new standards for speed, convenience, and affordability. It was also the first cycler small enough to be practical for field research. Technologically, it pioneered the use of Joule-assisted Peltier thermal control and precision thermistor sensors with NIST-traceable accuracy.
	1988 — PTC-100® Thermal Cycler The premier Peltier-effect thermal cycler — this compact and reliable instrument delivers precision and is easy to use. The novel Peltier heating and cooling technology established a widespread reputation for innovation and versatility.

Notice Regarding Bio-Rad Thermal Cyclers and Real-Time PCR Systems
Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and development and for use in human in vitro diagnostics and all other applied fields under U.S. Patent Number 5,475,610 (Claims 1, 44, 158, 160–163, and 167 only), or corresponding claims in its non-U.S. counterpart, owned by Applera Corporation. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5' nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Bio-Rad's real-time thermal cyclers are licensed real-time thermal cyclers under Applera's U.S. Patent Number 6,814,934 B1 for use in research, human in vitro diagnostics, and all other fields except veterinary diagnostics.

Bio-Rad's thermal cyclers and real-time thermal cyclers are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 6,767,512 and 7,074,367.

Roche Enzyme Products Conveying Polymerase Patent Rights
Use of this product is covered by one or more of the following U.S. patents and corresponding patent claims outside the U.S.: 5,789,224; 5,618,711; and 6,127,155; and claims outside the U.S. corresponding to U.S. Patent Number 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Licensed dsDNA–Binding Dye Research Kits
Use of this product is covered by one or more of the following U.S. patents and corresponding patent claims outside the U.S.: 5,994,056 and 6,171,785. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Thermal Cycler Authorization Notices
As a result of the license agreements entered between Bio-Rad Laboratories, Inc. and Applied Biosystems, all thermal cyclers and real-time instrument systems from Bio-Rad, including those previously sold by MJ Research or Bio-Rad, are authorized thermal cyclers and real-time systems. The following Authorization Notices describe the rights conveyed to the purchaser of such a thermal cycler or system.

Thermal Cycler Chassis with Real-Time Upgrade Option
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Thermal Cycler Chassis and Thermal Cyclers
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Real-Time Thermal Cyclers
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