See all your data on one gel, not two. An 18-well Criterion Tris-HCI 4-20% gradient gel is capable of running twice as many samples as a standard mini gel. Lanes 1 and 18, Precision Protein electrophoresis standards; lanes 2 and 10, chicken extract; lanes 3 and 11, shrimp extract; lanes 4 and 9 fish extract; lanes 5 and 13, ADH; lanes 6 and 16, catalase, lane 7, snake venom; lane 8, E coli lysate; lane 12, peroxidase; lane 14 raccoon serum; lane 15, urease; lane 17, myosin.
Mouse liver extract separated on an 11cm ReadyStrip IPG strip, pH 3–10, followed by second-dimension separationn on a 4-12% Criterion XT Bis-Tris gel with Precision Plus Protein standards. The gel was run with XT MOPS buffer and stained with SYPRO Ruby protein gel stain.