Gravity and Spin Chromatography Columns

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Overview

These empty and prepacked columns provide convenience for sample preparation and other small-scale applications.

Disposable Columns

Econo-Pac Columns

Guide to Bio-Spin Columns

Category Products

Empty Gravity Flow Columns
Empty Econo-Pac and Poly-Prep gravity-flow columns and accessories are ideal for sample and buffer preparation, as well as for gravity flow chromatography applications.

Empty Spin Columns
Empty Bio-Spin and Micro Bio-Spin chromatography columns are disposable polypropylene spin columns that can be packed with a variety of chromatographic media.

Bio-Spin and Micro Bio-Spin Size Exclusion Columns
Bio-Spin and Micro Bio-Spin size exclusion columns are ideal for quick, easy, and inexpensive cleanup of DNA or protein samples.

PCR Kleen™ Spin Columns
PCR Kleen columns are prepacked spin columns for purifying PCR products and other DNA molecules of >200 bp directly from reaction mixtures.

Prepacked Gravity Flow Columns for Ion Exchange
Poly-Prep prepacked columns for gravity-flow chromatography provide convenience for sample preparation and other small-scale applications. The graduated 0.8 x 4 cm columns are made of polypropylene.

Econo-Pac^® Protein A Prepacked Gravity Flow Columns
Econo-Pac prepacked columns for gravity-flow chromatography allow fast and simplified antibody purification.

Econo-Pac^® 10DG Desalting Prepacked Gravity Flow Columns
Econo-Pac prepacked columns for gravity-flow chromatography allow fast and easy desalting.

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Call us at 1-800-4-BIORAD (1-800-424-6723)

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	Bio-Spin 6	Micro Bio-Spin 6	Bio-Spin 30	Micro Bio-Spin 30
Packed support	Special grade Bio-Gel P-6 gel	Special grade Bio-Gel P-6 gel	Special grade Bio-Gel P-30 gel	Special grade Bio-Gel P-30 gel
Equilibration buffer	SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*	SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*
Applications	Desalting and buffer exchange	Desalting and buffer exchange	Desalting; nucleotide and small molecule removal	DNA sequencing reaction mixtures (Tris), and small molecule removal
Bed volume	1.1 ml	0.7 ml	1.1 ml	0.7 ml
Retention and recovery	90% recovery of 20 bases or bp, 99% retention of salts	90% recovery of 20 bases or bp, 99% retention of salts	95% recovery of 22 bases or bp, 98% retention of ddNTPs	95% recovery of 22 bases or bp, 98% retention of ddNTPs
MW exclusion limit, globular proteins	6,000	6,000	40,000	40,000
Sample volume	50–100 µl	10–75 µl	50–100 µl	10–75 µl
Centrifuge type	Swinging bucket	Microcentrifuge	Swinging bucket	Microcentrifuge
Autoclavability	Yes	Yes	Yes	Yes
* 150 mM NaCl, 17.5 mM sodium citrate, pH 7.0

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Gravity and Spin Chromatography Columns

Overview

Category Products

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Gravity and Spin Chromatography Columns

Overview

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