Pulsed Field Gel Electrophoresis Systems

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Overview

Three CHEF systems that incorporate different technologies for optimal resolution in various size ranges, such as PACE, FIGE, and AFIGE are offered. Agarose, reagents, standards, and kits for PFGE are available.

Introduction to PFGE

CHEF Mapper XA

Nonlinear Switch and Mobility

PFGE Standards Selection Guide

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CHEF Mapper^® XA System
The CHEF Mapper XA system incorporates patented FIGE and AFIGE technologies for superior resolution from 100 bp to 10 Mb, in addition to the features of the CHEF-DR II and the CHEF-DR III systems.

CHEF-DR^® III Variable Angle System
The CHEF-DR III variable angle system combines patented PACE technology and proven CHEF technology into one easy-to-use instrument.

CHEF-DR II System
The CHEF-DR II system has been streamlined by condensing the functions of Pulsewave 760 technology and the power supply into the CHEF-DR II control module.

CHEF Genomic DNA Plug Kits
CHEF genomic DNA plug kits provide a convenient means for preparing intact, chromosome-sized DNA for PFGE.

Pulsed Field Standards and Agaroses
Bio-Rad offers Certified megabase agarose, pulsed field Certified agarose, and standards for applications from FIGE separation of cosmid inserts to separation of the largest chromosomes.

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5 Mb Ladder	8–48 kb Ladder	Lambda Ladder	S. cerevisiae Chromosomal DNA	H. wingei Chromosomal DNA	S. pombe Chromosomal DNA

Comparison of Pulsed Field Standards

	Range	Contents	Amount	Number of Applications

5 kb ladder	4.9–120 kb	Concatemers of pBR328	20 µg in 200 µl	20–25
8–48 kb ladder	8.3–48.5 kb	Mixed digest of phage λ	25 µg in 125 µl	125
Lambda (λ) ladder	0.05–1 Mb	Concatemers of phage λcl857Sam7	5 agarose blocks	25–40
S. cerevisiae	0.225–2.2 Mb	Saccharomyces cerevisiae chromosomal DNA	5 agarose blocks	25–40
H. wingei	1–3.1 Mb	Hansenula wingei chromosomal DNA	5 agarose blocks	25–40
S. pombe	3.5–5.7 Mb	Schizosaccharomyces pombe chromosomal DNA	5 agarose blocks	25–40

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Pulsed Field Gel Electrophoresis Systems

Overview

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High-resolution separation with multiple states (pulse angles). S. cerevisiae chromosomes separated under two-state conditions (left) and under multistate conditions (right). Notice separation of the comigrating chromosomes under multistate conditions.	Increased separation with secondary pulsed field electrophoresis. S. cerevisiae chromosomes separated under two-state conditions (left) and under two-state conditions with secondary pulses (right).

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Pulsed Field Gel Electrophoresis Systems

Overview

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