Prepacked Bio-Scale™ Mini Cartridges Sampler Packs

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

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* Flow rate = 150 cm/hr; ** flow rate = 300 cm/hr

Comparison of Bio-Scale mini cartridges to other affinity columns on the Profinia™ protein purification system. Yield (A) and purity (B) of various proteins purified using either Bio-Scale™ Mini Profinity™ IMAC cartridges () or commercially available prepacked columns HisTrap FF () or HisTrap HP () on the Profinia system. Rec1, 75 kD; Fih1, 37 kD; DHFR, 25 kD; SME1, 11 kD.

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* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Analysis of plasmid DNA purified on UNOsphere Q support. Fractions were separated on a 0.8% agarose gel. Lane 1, 1 kb marker (catalog #170-8204); lane 2, crude lysate; lane 3, flowthrough (fractions 2–6); lane 4, fractions 9–18; lane 5, fractions 19–30; lane 6, fractions 31–35; lane 7, fraction 36.

Chromatogram of murine IgG1 purification on the UNOsphere S column during small-scale process development. Diluted cell culture (20 ml) was loaded onto an UNOsphere S 0.7 x 5 cm column at a flow rate of 600 cm/hr in 20 mM phosphate-citrate buffer, pH 4.0. The sample was eluted in 10 column volumes (CV) of a 0–0.5 M NaCl gradient, followed by 5 CV of 1 M NaCl in the same buffer. The column was then cleaned in 1 M NaOH. The double-headed arrow indicates the fractions (1 ml each) containing IgG1. Blue trace, A₂₈₀; red trace, conductivity profile.

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