Real-Time PCR Experimental Design - Citations
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Statistical aspects of quantitative real-time PCR experiment design
Methods PMID 20109551
Methods PMID 20109551
Author(s): Robert R Kitchen, Mikael Kubista, Ales Tichopad
Abstract: Experiments using quantitative real-time PCR to test hypotheses are limited by technical and biological variability; we seek to minimise sources of co... read more »
Abstract: Experiments using quantitative real-time PCR to test hypotheses are limited by technical and biological variability; we seek to minimise sources of co... read more »
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.
Audio, Transactions of the IRE Professional Group on PMID 19246619
Audio, Transactions of the IRE Professional Group on PMID 19246619
Author(s): Stephen A SA Bustin, Vladimir V Benes, Jeremy A JA Garson, Jan J Hellemans, Jim J Huggett, Mikael M Kubista, Reinhold R Mueller, Tania T Nolan, Michael W MW Pfaffl, Gregory L GL Shipley, Jo J Vandesompele, Carl T CT Wittwer
Abstract: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated b... read more »
Abstract: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated b... read more »
Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references.
J Exp Bot PMID 19264760
J Exp Bot PMID 19264760
Author(s): Stéphanie Guénin, Mélanie Mauriat, Jérôme Pelloux, Olivier Van Wuytswinkel, Catherine Bellini, Laurent Gutierrez
Abstract: Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-PCR or real-time RT-PCR) has been used in large proportions of... read more »
Abstract: Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-PCR or real-time RT-PCR) has been used in large proportions of... read more »
Statistical analysis of real-time PCR data.
BMC Bioinformatics PMID 16504059
BMC Bioinformatics PMID 16504059
Author(s): JoshuaS Yuan, Ann Reed, Feng Chen, C Neal Stewart
Abstract: Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time PCR a... read more »
Abstract: Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time PCR a... read more »
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.
Audio, Transactions of the IRE Professional Group on PMID 12184808
Audio, Transactions of the IRE Professional Group on PMID 12184808
Author(s): Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F
Abstract: Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of ch... read more »
Abstract: Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of ch... read more »
A new mathematical model for relative quantification in real-time RT-PCR.
Nucleic Acids Res PMID 11328886
Nucleic Acids Res PMID 11328886
Author(s): M W MW Pfaffl
Abstract: Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool i... read more »
Abstract: Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool i... read more »
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