MIQE and RDML Guidelines - Citations
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RDML: structured language and reporting guidelines for real-time quantitative PCR data.
Nucleic Acids Res PMID 19223324
Nucleic Acids Res PMID 19223324
Author(s): Steve S Lefever, Jan J Hellemans, Filip F Pattyn, Daniel R DR Przybylski, Chris C Taylor, René R Geurts, Andreas A Untergasser, Jo J Vandesompele,
Abstract: The XML-based Real-Time PCR Data Markup Language (RDML) has been developed by the RDML consortium (http://www.rdml.org) to enable straightforward exch... read more »
Abstract: The XML-based Real-Time PCR Data Markup Language (RDML) has been developed by the RDML consortium (http://www.rdml.org) to enable straightforward exch... read more »
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.
Audio, Transactions of the IRE Professional Group on PMID 19246619
Audio, Transactions of the IRE Professional Group on PMID 19246619
Author(s): Stephen A SA Bustin, Vladimir V Benes, Jeremy A JA Garson, Jan J Hellemans, Jim J Huggett, Mikael M Kubista, Reinhold R Mueller, Tania T Nolan, Michael W MW Pfaffl, Gregory L GL Shipley, Jo J Vandesompele, Carl T CT Wittwer
Abstract: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated b... read more »
Abstract: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated b... read more »
Unreliable Real-Time PCR Analysis of Human Endogenous Retrovirus-W (HERV-W) RNA Expression and DNA Copy Number in Multiple Sclerosis
AIDS Res Hum Retroviruses PMID 19292592
AIDS Res Hum Retroviruses PMID 19292592
Author(s): Jeremy A Garson, Jim F Huggett, Stephen A Bustin, Michael W Pfaffl, Vladimir Benes, Jo Vandesompele, Gregory L Shipley
The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.
Plant Biotechnol J PMID 18433420
Plant Biotechnol J PMID 18433420
Author(s): Laurent L Gutierrez, Mélanie M Mauriat, Stéphanie S Guénin, Jérôme J Pelloux, Jean-François JF Lefebvre, Romain R Louvet, Christine C Rusterucci, Thomas T Moritz, François F Guerineau, Catherine C Bellini, Olivier O Van Wuytswinkel
Abstract: Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. ... read more »
Abstract: Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. ... read more »
qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data.
Audio, Transactions of the IRE Professional Group on PMID 17291332
Audio, Transactions of the IRE Professional Group on PMID 17291332
Author(s): Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J
Abstract: Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing ... read more »
Abstract: Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing ... read more »
RNA integrity and the effect on the real-time qRT-PCR performance.
Mol Aspects Med PMID 16469371
Mol Aspects Med PMID 16469371
Author(s): Simone S Fleige, Michael W MW Pfaffl
Abstract: The assessment of RNA integrity is a critical first step in obtaining meaningful gene expression data. Working with low-quality RNA may strongly compr... read more »
Abstract: The assessment of RNA integrity is a critical first step in obtaining meaningful gene expression data. Working with low-quality RNA may strongly compr... read more »
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.
Audio, Transactions of the IRE Professional Group on PMID 12184808
Audio, Transactions of the IRE Professional Group on PMID 12184808
Author(s): Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F
Abstract: Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of ch... read more »
Abstract: Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of ch... read more »
- 1 - 7 of 7 results
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