Neural stem cell differentiation is mediated by integrin Î²4 in vitro
Le Su, Xin Lv, JiPing Xu, DeLing Yin, HaiYan Zhang, Yi Li, Jing Zhao, ShangLi Zhang, JunYing Miao
The International Journal of Biochemistry & Cell Biology
Neural stem cells are capable of differentiating into three major neural cell types, but the underlying molecular mechanisms remain unclear. Here, we investigated the mechanism by which integrin @b4 modulates mouse neural stem cell differentiation in vitro. Inhibition of endogenous integrin @b4 by RNA interference inhibited the cell differentiation and the expression of fibroblast growth factor receptor 2 but not fibroblast growth factor receptor 1 or fibroblast growth factor receptor 3. Overexpression of integrin @b4 in neural stem cells promoted neural stem cell differentiation. Furthermore, integrin @b4-induced differentiation of neural stem cells was attenuated by SU5402, the inhibitor of fibroblast growth factor receptors. Finally, we investigated the role of integrin @b4 in neural stem cell survival: knockdown of integrin @b4 did not affect survival or apoptosis of neural stem cells. These data provide evidence that integrin @b4 promotes differentiation of mouse neural stem cells in vitro possibly through fibroblast growth factor receptor 2.